中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2012年
46期
8583-8588
,共6页
任红革%李林%李振浩%孙培磊%崔逢德
任紅革%李林%李振浩%孫培磊%崔逢德
임홍혁%리림%리진호%손배뢰%최봉덕
骨性关节炎%基因芯片%膝关节%滑膜%信号转导%翻译调节%趋化作用%炎症应答%细胞增殖%差异表达
骨性關節炎%基因芯片%膝關節%滑膜%信號轉導%翻譯調節%趨化作用%炎癥應答%細胞增殖%差異錶達
골성관절염%기인심편%슬관절%활막%신호전도%번역조절%추화작용%염증응답%세포증식%차이표체
背景:原发性骨性关节炎的发展是多种相关基因表达失常的缘故.采用 mRNA 基因表达谱芯片技术,检测膝关节骨性关节炎滑膜差异表达基因,有助于阐明骨性关节炎病理过程的相关机制.目的:比较延边地区膝关节骨性关节炎和正常关节滑膜的基因表达谱变化,筛选出与膝关节骨性关节炎相关的差异表达基因,并探讨其在骨性关节炎发病机制中的意义.方法:选择延边地区原发性膝关节骨性关节炎患者9例及正常对照者3例,应用 mRNA 基因表达谱芯片技术检测所获得的滑膜组织中表达差异的基因,筛选出表达差异基因并对其进行 GO 分析.结果与结论:膝关节骨性关节炎及正常对照者滑膜的差异表达基因分析结果显示,延边地区膝关节骨关节炎滑膜中的差异表达基因共有1261个,其中上调的基因有451个,下调的基因有810个.差异表达基因 GO 统计分析显示,信号转导、翻译调节、趋化作用、炎症应答、细胞增殖等多种基因的表达有差异.基因芯片技术能有效地筛选出骨性关节炎差异表达的基因,并进一步证实了骨性关节炎的病理过程是多种功能基因参与的复杂的病理过程.
揹景:原髮性骨性關節炎的髮展是多種相關基因錶達失常的緣故.採用 mRNA 基因錶達譜芯片技術,檢測膝關節骨性關節炎滑膜差異錶達基因,有助于闡明骨性關節炎病理過程的相關機製.目的:比較延邊地區膝關節骨性關節炎和正常關節滑膜的基因錶達譜變化,篩選齣與膝關節骨性關節炎相關的差異錶達基因,併探討其在骨性關節炎髮病機製中的意義.方法:選擇延邊地區原髮性膝關節骨性關節炎患者9例及正常對照者3例,應用 mRNA 基因錶達譜芯片技術檢測所穫得的滑膜組織中錶達差異的基因,篩選齣錶達差異基因併對其進行 GO 分析.結果與結論:膝關節骨性關節炎及正常對照者滑膜的差異錶達基因分析結果顯示,延邊地區膝關節骨關節炎滑膜中的差異錶達基因共有1261箇,其中上調的基因有451箇,下調的基因有810箇.差異錶達基因 GO 統計分析顯示,信號轉導、翻譯調節、趨化作用、炎癥應答、細胞增殖等多種基因的錶達有差異.基因芯片技術能有效地篩選齣骨性關節炎差異錶達的基因,併進一步證實瞭骨性關節炎的病理過程是多種功能基因參與的複雜的病理過程.
배경:원발성골성관절염적발전시다충상관기인표체실상적연고.채용 mRNA 기인표체보심편기술,검측슬관절골성관절염활막차이표체기인,유조우천명골성관절염병리과정적상관궤제.목적:비교연변지구슬관절골성관절염화정상관절활막적기인표체보변화,사선출여슬관절골성관절염상관적차이표체기인,병탐토기재골성관절염발병궤제중적의의.방법:선택연변지구원발성슬관절골성관절염환자9례급정상대조자3례,응용 mRNA 기인표체보심편기술검측소획득적활막조직중표체차이적기인,사선출표체차이기인병대기진행 GO 분석.결과여결론:슬관절골성관절염급정상대조자활막적차이표체기인분석결과현시,연변지구슬관절골관절염활막중적차이표체기인공유1261개,기중상조적기인유451개,하조적기인유810개.차이표체기인 GO 통계분석현시,신호전도、번역조절、추화작용、염증응답、세포증식등다충기인적표체유차이.기인심편기술능유효지사선출골성관절염차이표체적기인,병진일보증실료골성관절염적병리과정시다충공능기인삼여적복잡적병리과정.
BACKGROUND: The progression of primary osteoarthritis is the result of abnormal expression of various genes. We use mRNA gene chip technique to detect the synovial gene expression of knee osteoarthritis, which provides evidence for related pathological mechanism of knee osteoarthritis. OBJECTIVE: To compare the alteration of synovial gene expression in patients with knee osteoarthritis and normal people in Yanbian area, and to evaluate the potential osteoarthritis related discriminating genes and their roles in pathological mechanism. METHODS: This study included nine patients with knee osteoarthritis and three healthy controls. mRNA gene chip technique was used to analyze the gene expression profile of synovial tissue in these subjects and GO analysis was performed. RESULTS AND CONCLUSION: The results of gene expression in the synovial tissue of osteoarthritis patients and healthy controls group showed that 1 261 genes were differently expressed, including 451 up-regulated genes and 810 down-regulated genes. The GO analysis showed that the gene expression about signal transduction, regulation of transcription, chemotaxis, inflammatory response, and cel proliferation was different. Gene chip technique is effective for screening associated genes, and the pathological progression of osteoarthritis is a complex progression which many genes are involved.