中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2012年
46期
8611-8614
,共4页
体外冲击波%软骨细胞%细胞增殖%Ⅱ型胶原%兔
體外遲擊波%軟骨細胞%細胞增殖%Ⅱ型膠原%兔
체외충격파%연골세포%세포증식%Ⅱ형효원%토
背景:关节软骨损伤后自身修复能力有限,体外冲击波可能提供一种高质量的修复关节软骨损伤并达到很好远期疗效的方法.目的:探讨体外冲击波对兔关节软骨细胞增殖及Ⅱ型胶原蛋白表达的影响.方法:酶消化法获得正常兔膝关节软骨细胞,培养并传代,实验 A、B、C 组分别用0.5×105,1.5×105,2.5×105 Pa等3种强度能量体外冲击波干预,空白对照组无任何干预措施.结果与结论:细胞生长曲线在第6天实验 B 组显著高于其他各组(P <0.05);ELISA 法检测实验 B 组Ⅱ型胶原 A 值与其他各组相比,显著升高(P <0.05);RT-PCR 法检测实验 B 组Ⅱ型胶原表达明显增强,与其他各组相比差异有显著性意义(P <0.05).提示适当能量强度及频次的体外冲击波刺激,能够明显促进软骨细胞的增殖及Ⅱ型胶原表达.
揹景:關節軟骨損傷後自身脩複能力有限,體外遲擊波可能提供一種高質量的脩複關節軟骨損傷併達到很好遠期療效的方法.目的:探討體外遲擊波對兔關節軟骨細胞增殖及Ⅱ型膠原蛋白錶達的影響.方法:酶消化法穫得正常兔膝關節軟骨細胞,培養併傳代,實驗 A、B、C 組分彆用0.5×105,1.5×105,2.5×105 Pa等3種彊度能量體外遲擊波榦預,空白對照組無任何榦預措施.結果與結論:細胞生長麯線在第6天實驗 B 組顯著高于其他各組(P <0.05);ELISA 法檢測實驗 B 組Ⅱ型膠原 A 值與其他各組相比,顯著升高(P <0.05);RT-PCR 法檢測實驗 B 組Ⅱ型膠原錶達明顯增彊,與其他各組相比差異有顯著性意義(P <0.05).提示適噹能量彊度及頻次的體外遲擊波刺激,能夠明顯促進軟骨細胞的增殖及Ⅱ型膠原錶達.
배경:관절연골손상후자신수복능력유한,체외충격파가능제공일충고질량적수복관절연골손상병체도흔호원기료효적방법.목적:탐토체외충격파대토관절연골세포증식급Ⅱ형효원단백표체적영향.방법:매소화법획득정상토슬관절연골세포,배양병전대,실험 A、B、C 조분별용0.5×105,1.5×105,2.5×105 Pa등3충강도능량체외충격파간예,공백대조조무임하간예조시.결과여결론:세포생장곡선재제6천실험 B 조현저고우기타각조(P <0.05);ELISA 법검측실험 B 조Ⅱ형효원 A 치여기타각조상비,현저승고(P <0.05);RT-PCR 법검측실험 B 조Ⅱ형효원표체명현증강,여기타각조상비차이유현저성의의(P <0.05).제시괄당능량강도급빈차적체외충격파자격,능구명현촉진연골세포적증식급Ⅱ형효원표체.
BACKGROUND: Injured articular cartilage has a very limited self-repair ability. Extracorporeal shock wave may provide a method that has high-quality repair of articular cartilage defects and can achieve good late outcomes. OBJECTIVE: To explore the effect of extracorporeal shock wave on the proliferation of rabbit articular chondrocytes and type Ⅱ colagen expression. METHODS: Normal rabbit knee joint chondrocytes were obtained by enzymatic digestion method. The cel s were cultured, passaged, and then randomized into three experimental groups: A, B and C groups were given 0.5×105, 1.5×105 and 2.5×105 Pa extracorporeal shock wave intervention, respectively. The blank control received no intervention. RESULTS AND CONCLUSION: The cel growth curve in the group B at day 6 was higher than that in the other groups (P< 0.05). Enzyme-linked immunosorbent assay showed that compared with the other groups, the absorbance of type Ⅱcol agen in the group B was significantly increased (P < 0.05). Reverse transcription PCR indicated that type expression was enhanced obviously in the group B, as compared with the other groups (P < 0.05). These results suggest that the extracorporeal shock wave stimulation of appropriate energy intensity and frequency can significantly promote chondrocyte proliferation and typeⅡ colagen expression.
