CAJ | 학술논문

背景:多种肿瘤中胰岛素样生长因子1受体及其配体表达异常,且胰岛素样生长因子1是多种细胞的有丝分裂原和抗细胞凋亡的因子,控制多种细胞的增殖、分化和凋亡的进程.目的:观察胰岛素样生长因子1及胰岛素样生长因子1受体在病理性瘢痕中的表达.方法:收集中山大学附属第一医院烧伤外科收治患者增生性瘢痕、瘢痕疙瘩及其正常皮肤等手术切除标本,荧光定量实时 PCR 检测不同组织中胰岛素样生长因子1及其受体 mRNA 的表达水平.结果与结论:瘢痕疙瘩及增生性瘢痕中胰岛素样生长因子1 mRNA 表达水平均高于正常皮肤(P <0.05),且在瘢痕疙瘩及增生性瘢痕组织中胰岛素样生长因子1 表达水平差异无显著性意义(P >0.05),胰岛素样生长因子1受体在增生性瘢痕表达水平与正常皮肤接近(P >0.05);而瘢痕疙瘩中胰岛素样生长因子1受体明显高于瘢痕周围正常皮肤(P <0.05).说明胰岛素样生长因子1及其受体在病理性瘢痕的形成过程中起重要作用.
배경:다충종류중이도소양생장인자1수체급기배체표체이상,차이도소양생장인자1시다충세포적유사분렬원화항세포조망적인자,공제다충세포적증식、분화화조망적진정.목적:관찰이도소양생장인자1급이도소양생장인자1수체재병이성반흔중적표체.방법:수집중산대학부속제일의원소상외과수치환자증생성반흔、반흔흘탑급기정상피부등수술절제표본,형광정량실시 PCR 검측불동조직중이도소양생장인자1급기수체 mRNA 적표체수평.결과여결론:반흔흘탑급증생성반흔중이도소양생장인자1 mRNA 표체수평균고우정상피부(P <0.05),차재반흔흘탑급증생성반흔조직중이도소양생장인자1 표체수평차이무현저성의의(P >0.05),이도소양생장인자1수체재증생성반흔표체수평여정상피부접근(P >0.05);이반흔흘탑중이도소양생장인자1수체명현고우반흔주위정상피부(P <0.05).설명이도소양생장인자1급기수체재병이성반흔적형성과정중기중요작용.
BACKGROUND: In many tumors, insulin-like growth factor 1 and its receptor express abnormal y. Insulin-like growth factor is the mitogen and anti-apoptotic factor of a variety of cel s, control ing the proliferation, differentiation and apoptosis of various cel s. OBJECTIVE: To observe the expression of insulin-like growth factor-1 and insulin-like growth factor -1 receptor in pathological scar. METHODS: Surgical specimens of hypertrophic scars, keloids and normal skin were col ected from the patients admitted in the Department of Burn Surgery, First Affiliated Hospital of Sun Yat-sen University. Fluorescent quantitative real-time PCR technique was used to detect the mRNA expression of insulin-like growth factor-1 and insulin-like growth factor -1 receptor in different tissues. RESULTS AND CONCLUSION: The mRNA expression of insulin-like growth factor-1 was higher in the hypertrophic scars and keloids than in the normal skin (P < 0.05). However, there was no difference in the mRNA expression of insulin-like growth factor-1 between the hypertrophic scars and keloids (P > 0.05). The expression of insulin-like growth factor-1 receptor in the hypertrophic scars was close to that in the normal skin (P > 0.05); while, the expression of insulin-like growth factor-1 receptor in the keloids was significantly higher than that in the surrounding tissues of keloids (P < 0.05). These findings indicate that insulin-like growth factor-1 and its receptor play an important role in the formation of pathological scars.