CAJ | 학술논문

背景:应力屏蔽引起的肌腱组织内各种细胞因子的改变与肌腱挛缩密切相关,其中转化生长因子β的改变情况尚不清楚.目的:观察应力屏蔽对跟腱转化生长因子β浓度的影响.方法:取雄性 SD 大鼠20只随机分为2组,左后肢行跟腱应力屏蔽后分别饲养2周和4周,随机抽取的10只大鼠的右后肢正常跟腱组织做对照.以 ELISA 定量检测各组转化生长因子β的质量浓度.结果与结论:造模组转化生长因子β均比正常组显著升高(P 均<0.01);造模2周与4周组间差异无显著性意义(P >0.05).结果表明跟腱应力屏蔽2周后转化生长因子β已明显升高,到屏蔽4周未见下降,肌腱的合成代谢增强.
배경:응력병폐인기적기건조직내각충세포인자적개변여기건련축밀절상관,기중전화생장인자β적개변정황상불청초.목적:관찰응력병폐대근건전화생장인자β농도적영향.방법:취웅성 SD 대서20지수궤분위2조,좌후지행근건응력병폐후분별사양2주화4주,수궤추취적10지대서적우후지정상근건조직주대조.이 ELISA 정량검측각조전화생장인자β적질량농도.결과여결론:조모조전화생장인자β균비정상조현저승고(P 균<0.01);조모2주여4주조간차이무현저성의의(P >0.05).결과표명근건응력병폐2주후전화생장인자β이명현승고,도병폐4주미견하강,기건적합성대사증강.
BACKGROUND: The changes of cytokines induced by stress shielding in the tendon are closely related to tendon contracture. But the situation of transformation growth factor (TGF)-β remains unclear. OBJECTIVE: To investigate the effect of stress shielding on TGF-β concentration. METHODS: Twenty mature male SD rats were randomly divided into model and control groups. Rats in the model group were undergone stress shielding of the Achil es tendon in the left hind limb, and then rose for 2 and 4 weeks. Randomly selected 10 rats’ normal Achil es tendons in the right hind limb served as controls. The levels of TGF-β in the two groups were tested by detection of enzyme-linked immunosorbent assay. RESULTS AND CONCLUSION: The level of TGF-β in the model group was higher than that in the control groups (P <0.01). There was no difference between the 2 weeks model group and 4 weeks model group (P > 0.05). These results suggest that after stress shielding of the Achil es tendon for 2 weeks, the level of TGF-β increases obviously and does not decrease at week 4, moreover, the anabolism of tendon is enhanced.