CAJ | 학술논문

背景:张力在细胞增殖、分化、凋亡以及基因表达过程中起着重要的作用.目的:观察张力对小鼠增生性瘢痕形成及羟脯氨酸含量的影响.方法:4周龄大 C57/BL 小鼠随机分为2组,张力组小鼠背部制作一个2 cm 长直线性全层皮肤切口,用尼龙线缝合,4 d 后拆线,再用尼龙线将22-mm 扩张螺丝固定到已经愈合的创面上,隔日扩张.对照组扩张螺丝不动.分别于伤后1,2,3,4,5周各取6只小鼠瘢痕组织观察瘢痕组织厚度、横截面积改变,测量瘢痕组织羟脯氨酸含量.结果与结论:张力组瘢痕增生明显,其厚度、横截面积显著大于对照组(P <0.01),张力组各时间点瘢痕组织羟脯氨酸含量显著高于对照组(P <0.01).表明张力导致小鼠瘢痕增生显著,羟脯氨酸含量显著增高.
배경:장력재세포증식、분화、조망이급기인표체과정중기착중요적작용.목적:관찰장력대소서증생성반흔형성급간포안산함량적영향.방법:4주령대 C57/BL 소서수궤분위2조,장력조소서배부제작일개2 cm 장직선성전층피부절구,용니룡선봉합,4 d 후탁선,재용니룡선장22-mm 확장라사고정도이경유합적창면상,격일확장.대조조확장라사불동.분별우상후1,2,3,4,5주각취6지소서반흔조직관찰반흔조직후도、횡절면적개변,측량반흔조직간포안산함량.결과여결론:장력조반흔증생명현,기후도、횡절면적현저대우대조조(P <0.01),장력조각시간점반흔조직간포안산함량현저고우대조조(P <0.01).표명장력도치소서반흔증생현저,간포안산함량현저증고.
BACKGROUND: Mechanical strain plays a key role in cel proliferation, differentiation, apoptosis and gene expression. OBJECTIVE: To investigate mechanical strain effects on the formation of hypertrophic scar and hydroxyproline content in mice. METHODS: Four weeks old C57/BL mice were randomly divided into mechanical strain group and control group. The mice in the mechanical strain group were subjected to a 2-cm ful -thickness skin incision, and then sutured with nylon wire. After stitches were taken out at day 4, 222-cm-mm stretching screw was fixed on the healed wound with nylon wire and expanded every other day. While stretching screw in the control group had no treatment. Six mice in each group were col ected respectively at weeks 1, 2, 3, 4 and 5 after wounded, and then their hypertrophic scar thickness, cross-sectional area changes and hydroxyproline content in the hypertrophic scar tissues were observed. RESULTS AND CONCLUSION: Compared with the control group, scar hyperplasia in the mechanical strain group was more obvious, besides, the thickness, cross-sectional area of hypertrophic scars was bigger (P < 0.01). Hydroxyproline content in the hypertrophic scar tissues of the mechanical strain group was significantly higher than that in the control group at each time point (P < 0.01). These results suggest that mechanical strain can result in obvious scar hyperplasia in mice and a significant increase in hydroxyproline content.