CAJ | 학술논문

背景:实验发现链球菌溶血素 O 引起的细胞通透在一定程度上是可逆的,链球菌溶血素 O 产生的孔道在 Ca2+存在的情况下可以自然封闭.目的:观察链球菌溶血素 O 是否可以使猪成纤维细胞的通透性发生改变,及不同质量浓度的链球菌溶血素 O 对细胞状态的影响.方法:分别使用质量浓度为0,230,300,400μg/L 的链球菌溶血素 O 溶液处理猪成纤维细胞,孵育50 min 后,加入 PI 染液,观察细胞的通透率.加入含 Ca2+的培养液封闭细胞后,分别观察细胞的形态变化及增殖能力的变化.结果与结论:随着链球菌溶血素 O 溶液质量浓度的增加,细胞的通透率只轻度提高.链球菌溶血素 O 处理细胞后,细胞的形态没有发生明显的改变.质量浓度为230μg/L 的链球菌溶血素 O 溶液对细胞的增殖能力无明显影响,而300μg/L 和400μg/L 的链球菌溶血素 O 溶液明显地降低了细胞的增殖能力.提示质量浓度为230μg/L 的链球菌溶血素 O 溶液可以提高细胞的通透性,并保持细胞正常的形态及增殖能力.
배경:실험발현련구균용혈소 O 인기적세포통투재일정정도상시가역적,련구균용혈소 O 산생적공도재 Ca2+존재적정황하가이자연봉폐.목적:관찰련구균용혈소 O 시부가이사저성섬유세포적통투성발생개변,급불동질량농도적련구균용혈소 O 대세포상태적영향.방법:분별사용질량농도위0,230,300,400μg/L 적련구균용혈소 O 용액처리저성섬유세포,부육50 min 후,가입 PI 염액,관찰세포적통투솔.가입함 Ca2+적배양액봉폐세포후,분별관찰세포적형태변화급증식능력적변화.결과여결론:수착련구균용혈소 O 용액질량농도적증가,세포적통투솔지경도제고.련구균용혈소 O 처리세포후,세포적형태몰유발생명현적개변.질량농도위230μg/L 적련구균용혈소 O 용액대세포적증식능력무명현영향,이300μg/L 화400μg/L 적련구균용혈소 O 용액명현지강저료세포적증식능력.제시질량농도위230μg/L 적련구균용혈소 O 용액가이제고세포적통투성,병보지세포정상적형태급증식능력.
BACKGROUND: Streptolysin O (SLO)-induced cel permeability is reversible to a certain extent, and the pores can be closed in the medium containing Ca2+. OBJECTIVE: To observe whether SLO could improve the permeability of pig fibroblasts and to observe the states of cel s treated with SLO of different concentrations. METHODS: Pig fibroblasts were permeated with SLO solution of different concentrations (0, 230, 300 and 400 μg/L respectively). After incubation of 50 minutes, the cel s were stained with PI to observe cel permeability. When adding the medium containing Ca2+, the cel s were plated. Morphology and multiplication capacity of these cel s were detected. RESULTS AND CONCLUSION: Along with increase of SLO solution concentration, the cel permeability improved slightly. When cel s were treated with SLO, the cel morphology was not changed. 230 μg/L SLO had no influence on the capacity of cel proliferation, and however, 300 μg/L and 400 μg/L SLO reduced the proliferation capacity significantly appearing statistical y difference when compared with 0 μg/L group. These findings indicate that 230 μg/L SLO could increase cel permeability with keeping the normal shape and proliferation ability of cel s.