CAJ | 학술논문

背景:低氧诱导因子1α调节一些增加抗低氧耐受的基因的表达.氯化钴是一种能够稳定低氧诱导因子1α的化学试剂.目的:检测氯化钴预处理对急性重复低氧小鼠的影响.方法:Balb/C 小鼠随机分为预处理组和对照组,实验前3 h,两组分别注射氯化钴和生理盐水后,分别进行0次(正常对照组),1次,4次缺氧暴露,随即分别取海马组织进行指标检测.结果与结论:预处理组缺氧暴露 1次对低氧耐受时间显著高于对照组缺氧暴露 1次,预处理组缺氧暴露 1次,4次低氧耐受时间差异无显著性意义.对照组中缺氧暴露 4次组低氧诱导因子1 的 DNA 结合活力显著高于缺氧暴露 1次组和正常对照组,组间比较差异有显著性意义.预处理组中缺氧暴露 1次组低氧诱导因子1 的 DNA 结合活力显著高于正常对照组(P <.01),缺氧暴露 4次组低氧诱导因子1 的 DNA 结合活力急剧下降,显著低于缺氧暴露 1次组和正常对照组.对照组促红细胞生成素和血管内皮生长因子 mRNA 在缺氧暴露 1次组和缺氧暴露 4次组升高,各组间比较差异有显著性意义(P <0.05).预处理组促红细胞生成素和血管内皮生长因子 mRNA 表达组间比较差异无显著性意义.提示氯化钴预处理能够提高低氧耐受时间,降低低氧预适应诱导的耐受时间及低氧诱导因子1 DNA 结合能力.
배경:저양유도인자1α조절일사증가항저양내수적기인적표체.록화고시일충능구은정저양유도인자1α적화학시제.목적:검측록화고예처리대급성중복저양소서적영향.방법:Balb/C 소서수궤분위예처리조화대조조,실험전3 h,량조분별주사록화고화생리염수후,분별진행0차(정상대조조),1차,4차결양폭로,수즉분별취해마조직진행지표검측.결과여결론:예처리조결양폭로 1차대저양내수시간현저고우대조조결양폭로 1차,예처리조결양폭로 1차,4차저양내수시간차이무현저성의의.대조조중결양폭로 4차조저양유도인자1 적 DNA 결합활력현저고우결양폭로 1차조화정상대조조,조간비교차이유현저성의의.예처리조중결양폭로 1차조저양유도인자1 적 DNA 결합활력현저고우정상대조조(P <.01),결양폭로 4차조저양유도인자1 적 DNA 결합활력급극하강,현저저우결양폭로 1차조화정상대조조.대조조촉홍세포생성소화혈관내피생장인자 mRNA 재결양폭로 1차조화결양폭로 4차조승고,각조간비교차이유현저성의의(P <0.05).예처리조촉홍세포생성소화혈관내피생장인자 mRNA 표체조간비교차이무현저성의의.제시록화고예처리능구제고저양내수시간,강저저양예괄응유도적내수시간급저양유도인자1 DNA 결합능력.
BACKGROUND: Hypoxia-inducible factor-1 α (HIF-1α) regulates the expression of genes which increase resistance to hypoxia tolerance. CoCl2 is a chemical reagent that can stabilize HIF-1α. OBJECTIVE: To detect the effects of CoCl2 pretreatment on acute repetitive hypoxic exposure of mice. METHODS: Balb/c mice were randomly divided into chemical pretreatment group and normal group. At 3 hours before treatment, the mice in the two groups were respectively injected with CoCl2 and normal saline. After that, the mice in the two groups were subjected to hypoxic exposure for 0 run (normal control group), 1 run, and 4 runs, respectively. Subsequently, hippocampi of mice were removed immediately after the exposure for index detection. RESULTS AND CONCLUSION: The tolerance time after one-time hypoxic exposure in the chemical pretreatment group was higher than that in the control group, but there was no significant difference in four times hypoxic exposure between the chemical pretreatment and control groups. HIF-1 DNA binding activity of the four times hypoxic exposure subgroup in the control group was significantly higher than that of the one-time hypoxic exposure subgroup in the chemical pretreatment group and the normal control group. There was significant difference in each group. HIF-1 DNA binding activity of the one-time hypoxic exposure subgroup in the chemical pretreatment group was obviously higher than that in the normal group (P < 0.01). In the control group, erythropoietin and vascular endothelial growth factor mRNA level in the one-time hypoxic exposure and four times hypoxic exposure subgroups showed increased. Each group had significant difference (P < 0.05). There was no significant difference in erythropoietin and vascular endothelial growth factor mRNA levels among the chemical pretreatment group. These results suggest that CoCl2 chemical pretreatment can improve the tolerance time in hypoxic exposure, and thereby reduce induction of tolerance after hypoxic preconditioning and HIF-1 DNA binding activity.