CAJ | 학술논문

背景:同种异体脱脂、脱蛋白松质骨具有与受体相同的三维立体结构,力学性能稳定,排异反应弱,细胞相容性好等独特的生物学性能.目的:通过理化方法制备同种异体脱脂、脱蛋白松质骨支架材料,分析其理化特性.方法:剥离兔髂骨10对,制作成约1.0 cm×0.8 cm×0.1 cm 的骨条,经脱脂、脱蛋白、深低温冷冻处理制备骨支架材料,检测其生物化学性能.测定支架材料与骨髓间充质干细胞的黏附率;将支架植入同种属动物体内,观察其组织相容性、免疫反应.结果与结论:同种异体脱脂、脱蛋白松质骨支架材料保留了天然骨组织的网状孔隙结构,孔隙率为(80.23±5.65)%,孔径最大为(318.11±17.51)μm,最小为(209.37±11.33)μm.骨髓间充质干细胞不仅能与支架黏附,而且能在支架上分裂、增殖.兔体内植入6周后支架周围界面未引起明显的炎症和排斥反应,并形成少量骨样组织.说明脱脂、脱蛋白松质骨支架具有适宜的三维多孔结构,与种子细胞黏附率高,有良好的生物相容性和细胞-材料界面作用;同时有一定的成骨作用.
배경:동충이체탈지、탈단백송질골구유여수체상동적삼유입체결구,역학성능은정,배이반응약,세포상용성호등독특적생물학성능.목적:통과이화방법제비동충이체탈지、탈단백송질골지가재료,분석기이화특성.방법:박리토가골10대,제작성약1.0 cm×0.8 cm×0.1 cm 적골조,경탈지、탈단백、심저온냉동처리제비골지가재료,검측기생물화학성능.측정지가재료여골수간충질간세포적점부솔;장지가식입동충속동물체내,관찰기조직상용성、면역반응.결과여결론:동충이체탈지、탈단백송질골지가재료보류료천연골조직적망상공극결구,공극솔위(80.23±5.65)%,공경최대위(318.11±17.51)μm,최소위(209.37±11.33)μm.골수간충질간세포불부능여지가점부,이차능재지가상분렬、증식.토체내식입6주후지가주위계면미인기명현적염증화배척반응,병형성소량골양조직.설명탈지、탈단백송질골지가구유괄의적삼유다공결구,여충자세포점부솔고,유량호적생물상용성화세포-재료계면작용;동시유일정적성골작용.
BACKGROUND: Defatted and deproteinsed cancel ous bone al ograft has good biological properties. It has a three-dimensional structure that is the same as the recipient’s, stable mechanical properties, weak rejection and excel ent cytocompatibility. OBJECTIVE: To prepare defatted and deproteinsed cancel ous bone al ograft scaffold materials by physiochemical methods, and to analyze physical and chemical characteristics. METHODS: Healthy 6-month-old New Zealand white rabbits were chosen, and 20 iliac bones were dissected into 1.0 cm×0.8 cm×0.1 cm bone. Bone scaffold material was prepared by defatting, deproteinization, as wel as low and depths freezing temperatures. Its physical and chemical characteristics were tested, and the adhesion rate of the scaffold material and bone marrow stromal stem cel s was detected. The scaffold was implanted into the same animal species, and histocompatibility and immunoreaction were observed. RESULTS AND CONCLUSION: The scaffold materials reserved the mesh pore structures of natural bone tissues with the pore ratio of (80.23±5.65)%. Maximum aperture was (318.112±17.51) μm, and minimum aperture was (209.37±11.33) μm. Bone marrow stromal stem cel s could not only adhere to the scaffold, but also divide and proliferate on the scaffold. After implantation for 6 weeks, there were no obvious inflammation and rejection around the scaffold interface and a smal amount of bone-like tissues formed. These results suggest that the deffatted and deproteinsed cancel ous bone scaffold has the appropriate three-dimensional porous structure, and adhesion rate of seed cel s is high, besides, it has good biocompatibility and cel -material interface role; at the same time there is a certain osteogenesis function.