CAJ | 학술논문

背景:课题组运用发酵技术开发出了一种新型多聚羟基烷酸——羟基丁酸与羟基辛酸共聚物,不仅具有聚羟基烷酸的通性,而且其柔韧性与加工性能得到较大改善.目的:检测羟基丁酸与羟基辛酸共聚物载碱性成纤维细胞生长因子纳米微球对骨髓间充质干细胞增殖活性的影响.方法:采用 W1/O/W2超声乳化法制备羟基丁酸与羟基辛酸共聚物载碱性成纤维细胞生长因子纳米微球,采用全骨髓法培养骨髓间充质干细胞,按照培养液中所含成分不同分为3组:碱性成纤维细胞生长因子组、纳米微球组、对照组,其中前两组碱性成纤维细胞生长因子的有效质量浓度分别设为10,20,50μg/L.结果与结论:共培养第1,3天,碱性成纤维细胞生长因子组与纳米微球组吸光度值比较差异无显著性意义(P >0.05),但吸光度值显著高于对照组(P <0.05),即碱性成纤维细胞生长因子对骨髓间充质干细胞具有明显促增殖作用;第5,7天纳米微球组吸光度值高于碱性成纤维细胞生长因子组(P <0.01),即纳米微球缓慢释放碱性成纤维细胞生长因子,明显提高生物利用度;第10天纳米微球组细胞仍然有较强的增殖能力,与其他2组比较差异有显著性意义(P <0.01),而此时碱性成纤维细胞生长因子组、对照组间差异已无显著性意义(P >0.05).结果说明纳米微球对碱性成纤维细胞生长因子具有良好的缓释作用,能发挥较为持久的生物学效应,可以持续促进骨髓间充质干细胞增殖.
배경:과제조운용발효기술개발출료일충신형다취간기완산——간기정산여간기신산공취물,불부구유취간기완산적통성,이차기유인성여가공성능득도교대개선.목적:검측간기정산여간기신산공취물재감성성섬유세포생장인자납미미구대골수간충질간세포증식활성적영향.방법:채용 W1/O/W2초성유화법제비간기정산여간기신산공취물재감성성섬유세포생장인자납미미구,채용전골수법배양골수간충질간세포,안조배양액중소함성분불동분위3조:감성성섬유세포생장인자조、납미미구조、대조조,기중전량조감성성섬유세포생장인자적유효질량농도분별설위10,20,50μg/L.결과여결론:공배양제1,3천,감성성섬유세포생장인자조여납미미구조흡광도치비교차이무현저성의의(P >0.05),단흡광도치현저고우대조조(P <0.05),즉감성성섬유세포생장인자대골수간충질간세포구유명현촉증식작용;제5,7천납미미구조흡광도치고우감성성섬유세포생장인자조(P <0.01),즉납미미구완만석방감성성섬유세포생장인자,명현제고생물이용도;제10천납미미구조세포잉연유교강적증식능력,여기타2조비교차이유현저성의의(P <0.01),이차시감성성섬유세포생장인자조、대조조간차이이무현저성의의(P >0.05).결과설명납미미구대감성성섬유세포생장인자구유량호적완석작용,능발휘교위지구적생물학효응,가이지속촉진골수간충질간세포증식.
BACKGROUND: A new-type poly 3-hydroxybutyrate-co-3-hydroxyoctanoate (PHBHOx) has been developed by fermentation technology, which not only possesses general characteristics but also has impnoued flexibility and processing performance. OBJECTIVE: To detect the effect of PHBHOx encapsulated basic fibroblast growth factor (bFGF) nanospheres on the proliferation of bone mesenchymal stem cel s (BMSCs). METHODS: PHBHOx encapsulated bFGF nanospheres were prepared by W1/O/W2 ultrasound emulsification method. Then, BMSCs were cultured by whole bone marrow method. After that the culture solution was divided into three groups: bFGF group, nanospheres group and control group according to its different ingredients. The effective concentration of bFGF in the former two groups was set to 10, 20 and 50 μg/L, respectively. RESULTS AND CONCLUSION: At days 1 and 3, there was no significant difference in absorbance value between bFGF group and nanospheres group (P > 0.05), but their absorbance value was both higher than that of the control group (P < 0.05); that is bFGF had promotive effect on BMSCs proliferation. At days 5 and 7, the absorbance value of the nanospheres group was higher than that of the bFGF group (P < 0.01), that was nanospheres could slowly relieve bFGF and obviously improve bioavailability. At day 10, nanospheres group showed strong proliferation capacity, and there was significant difference between the nanospheres group and the other two groups (P < 0.01). Meanwhile, bFGF group and control group had no significant difference (P > 0.05). These results suggest that there is a good sustained-release effect of nanospheres on bFGF and can play durable biological effects to promote BMSCs proliferation continual y.