CAJ | 학술논문

背景:研究表明使用生长因子直接或间接刺激新生血管形成可以促进皮瓣远端缺血部分的成活.目的:观察碱性成纤维细胞生长因子-聚乳酸-聚羟基乙酸共聚物缓释微球对家兔侧腹制作跨区轴型皮瓣成活的影响.方法:取24只健康家兔制作侧腹壁跨区轴型皮瓣,随机分组:实验组术前5 d 皮内注入碱性成纤维细胞生长因子-聚乳酸-聚羟基乙酸共聚物微球,对照组术前5 d 皮内注入碱性成纤维细胞生长因子+空微球悬浊液,空白对照组术前5 d 皮内注入生理盐水.5 d 后掀起皮瓣原位缝合.结果与结论:①皮瓣成活率:实验组显著高于对照组和空白对照组(P <0.01),②皮瓣组织学变化:实验组新生血管增生明显,以毛细血管为主.③CD34+免疫组织化学结果:实验组新生血管大量形成,平均血管数目高于对照组和空白对照组(P <0.05).表明术前5 d 局部注射碱性成纤维细胞生长因子-聚乳酸-聚羟基乙酸共聚物缓释球可促进皮瓣新生血管形成,增加皮瓣血运,促进皮瓣成活.
배경:연구표명사용생장인자직접혹간접자격신생혈관형성가이촉진피판원단결혈부분적성활.목적:관찰감성성섬유세포생장인자-취유산-취간기을산공취물완석미구대가토측복제작과구축형피판성활적영향.방법:취24지건강가토제작측복벽과구축형피판,수궤분조:실험조술전5 d 피내주입감성성섬유세포생장인자-취유산-취간기을산공취물미구,대조조술전5 d 피내주입감성성섬유세포생장인자+공미구현탁액,공백대조조술전5 d 피내주입생리염수.5 d 후흔기피판원위봉합.결과여결론:①피판성활솔:실험조현저고우대조조화공백대조조(P <0.01),②피판조직학변화:실험조신생혈관증생명현,이모세혈관위주.③CD34+면역조직화학결과:실험조신생혈관대량형성,평균혈관수목고우대조조화공백대조조(P <0.05).표명술전5 d 국부주사감성성섬유세포생장인자-취유산-취간기을산공취물완석구가촉진피판신생혈관형성,증가피판혈운,촉진피판성활.
BACKGROUND: Studies have shown that directly or indirectly stimulating angiogenesis can promote the survival of distal ischemia in part. OBJECTIVE: To investigate the effect of basic fibroblast growth factor (bFGF)-poly (lactic acid-glycolic acid) (PLGA)-sustained release microspheres on the survival of a cross-area flap on the lateral region of the rabbit abdomen. METHODS: Twenty-four health rabbits were col ected and the cross-area flap of rabbits was produced. Then the rabbits were randomly divided into three groups: experimental group, control group and blank control group. Five days before operation, rabbits in the experimental group were intradermal y injected with bFGF-PLGA microspheres, and those in the control group were intradermal y injected with bFGF+empty microsphere suspension; while those in the blank control group were intradermal y injected with normal saline. After 5 days, the skin flap was sutured. RESULTS AND CONCLUSION: (1)The skin flap survival rate of the experimental group was significantly higher than that of the control and blank control groups (P < 0.01). (2)Histological changes of skin flap tissues: A large number of granulation tissue formed in the experimental group, and neovascularization was obvious. (3)CD34+ immunohistochemistry results: A large number of new vessels were found in the experimental group. In addition, the average number of blood vessels was higher in the experimental group than in the control group and blank control group (P < 0.05). These findings suggest that the local injections of bFGF-PLGA sustained-release microspheres at 5 days preoperatively can promote skin flap neovascularization, increase the skin flap blood supply and promote skin flap survival.