CAJ | 학술논문

背景:去细胞异体神经移植物具有完全仿真的三维结构,为细胞附着提供了较理想的三维空间,是目前所有人工合成材料所无法比拟的.目的:对比分析液氮冷冻法、化学法及改良法3种方法对大鼠坐骨神经去细胞化的优劣.方法:切取 SD 大鼠10 mm 坐骨神经,分别以液氮冻融法、TritonX-100处理化学去细胞法、改良法处理的化学去细胞法分别制作去细胞神经支架,行苏木精-伊红染色,并在扫描电镜下观察支架超微结构.结果与结论:液氮冻融法去细胞支架内有较多许旺细胞残留,有较多轴突及髓鞘,基膜完整,基膜管欠通畅;TritonX-100处理化学法去细胞神经支架内无明显许旺细胞,管内未见明显轴突及髓鞘残留,基膜完整,基膜管通畅;改良法处理的化学去细胞神经支架内许旺细胞及轴突、髓鞘成分几乎完全消失,去细胞效果更好,基膜完整,基膜管更通畅.表明 TritonX-100处理化学去细胞法、改良法处理的化学去细胞法脱细胞效果较好,其中以改良法处理的化学去细胞法最好.
배경:거세포이체신경이식물구유완전방진적삼유결구,위세포부착제공료교이상적삼유공간,시목전소유인공합성재료소무법비의적.목적:대비분석액담냉동법、화학법급개량법3충방법대대서좌골신경거세포화적우렬.방법:절취 SD 대서10 mm 좌골신경,분별이액담동융법、TritonX-100처이화학거세포법、개량법처리적화학거세포법분별제작거세포신경지가,행소목정-이홍염색,병재소묘전경하관찰지가초미결구.결과여결론:액담동융법거세포지가내유교다허왕세포잔류,유교다축돌급수초,기막완정,기막관흠통창;TritonX-100처이화학법거세포신경지가내무명현허왕세포,관내미견명현축돌급수초잔류,기막완정,기막관통창;개량법처리적화학거세포신경지가내허왕세포급축돌、수초성분궤호완전소실,거세포효과경호,기막완정,기막관경통창.표명 TritonX-100처이화학거세포법、개량법처리적화학거세포법탈세포효과교호,기중이개량법처리적화학거세포법최호.
BACKGROUND: Acel ular al ogeneic nerve graft has a three-dimensional structure which is emulational completely, and provides an ideal three-dimensional space for cel adhesion. These are unmatchable for al synthetics at present. OBJECTIVE: To contrast and analyze the pro and con of the liquid nitrogen cryopreservation, chemical method and improved chemical method for preparation of acel ular sciatic nerve. METHODS: 10-mm sciatic nerve segments of SD rats were selected, and treated with liquid nitrogen cryopreservation, TritonX-100 chemical, and improved chemical methods. The acel ular nerve grafts were prepared for hematoxylin-eosin staining. The ultrastructure of the grafts was observed under the scanning electron microscopy. RESULTS AND CONCLUSION: Acel ular nerve grafts prepared by liquid nitrogen cryopreservation were found more residual schwann cel s, more axons and myelin. Grafts prepared by chemical method with TritonX-100 had no various schwann cel s, as wel as no significant residual axons and myelin were seen; the inophragma was intact and inophragma tube was clear. In the acel ular nerve grafts prepared by improved chemical method, Schwann cel s, axons and myelin almost disappeared; the acel ular effect was better, and the inophragma was intact, besides, nophragma tube was clearer. These findings suggest that the acel ular effects of chemical method and improved chemical method are better, and the improved chemical method is the best.