CAJ | 학술논문

背景:前期实验显示,在体外乙交酯-丙交酯共聚物支架与神经干细胞和许旺细胞有良好的相容性.目的:观察与许旺细胞共移植,神经干细胞是否能在乙交酯-丙交酯共聚物取向支架内存活、分化,乙交酯-丙交酯共聚物组织工程复合物是否能促进轴突再生及其髓鞘化.方法:制作成年 Wistar 大鼠 T8段半横断脊髓损伤模型,随机分为3组:支架组植入乙交酯-丙交酯共聚物支架,神经干细胞组植入接种神经干细胞(标记绿色荧光蛋白)的乙交酯-丙交酯共聚物取向支架,联合组植入接种神经干细胞(标记绿色荧光蛋白)和许旺细胞的乙交酯-丙交酯共聚物取向支架.结果与结论:移植的神经干细胞可在大鼠脊髓内存活,并迁移至邻近脊髓,联合组标记绿色荧光蛋白阳性细胞存活率显著高于神经干细胞组(P <0.001).联合组胶质纤维酸性蛋白/标记绿色荧光蛋白双阳性细胞多于神经元特异性烯醇化酶/标记绿色荧光蛋白双阳性细胞,神经干细胞组未发现神经元特异性烯醇化酶/标记绿色荧光蛋白双阳性细胞.联合组有少部分绿色荧光蛋白阳性细胞表达突触素,再生轴突和有髓轴突数量高于其他两组,但差异无显著性意义(P=0.058).表明与许旺细胞共移植,可促进神经干细胞向神经元样细胞分化,少部分神经元样细胞还可能形成了突触连接;种植了神经干细胞和许旺细胞的乙交酯-丙交酯共聚物支架可促进轴突再生及其髓鞘化.
배경:전기실험현시,재체외을교지-병교지공취물지가여신경간세포화허왕세포유량호적상용성.목적:관찰여허왕세포공이식,신경간세포시부능재을교지-병교지공취물취향지가내존활、분화,을교지-병교지공취물조직공정복합물시부능촉진축돌재생급기수초화.방법:제작성년 Wistar 대서 T8단반횡단척수손상모형,수궤분위3조:지가조식입을교지-병교지공취물지가,신경간세포조식입접충신경간세포(표기록색형광단백)적을교지-병교지공취물취향지가,연합조식입접충신경간세포(표기록색형광단백)화허왕세포적을교지-병교지공취물취향지가.결과여결론:이식적신경간세포가재대서척수내존활,병천이지린근척수,연합조표기록색형광단백양성세포존활솔현저고우신경간세포조(P <0.001).연합조효질섬유산성단백/표기록색형광단백쌍양성세포다우신경원특이성희순화매/표기록색형광단백쌍양성세포,신경간세포조미발현신경원특이성희순화매/표기록색형광단백쌍양성세포.연합조유소부분록색형광단백양성세포표체돌촉소,재생축돌화유수축돌수량고우기타량조,단차이무현저성의의(P=0.058).표명여허왕세포공이식,가촉진신경간세포향신경원양세포분화,소부분신경원양세포환가능형성료돌촉련접;충식료신경간세포화허왕세포적을교지-병교지공취물지가가촉진축돌재생급기수초화.
BACKGROUND: Previous studies have shown that poly (lactide-co-glycolide) scaffolds can exhibit good biocompatibility with neural stem cel s and Schwann cel s in vitro. OBJECTIVE: To investigate whether cografted with Schwann cel s, neural stem cel s can survive and differentiate in poly (lactide-co-glycolide) scaffold, and whether poly (lactide-co-glycolide) tissue-engineering complexes can promote axonal regeneration and myelinization. METHODS: A Wistar rat model of spinal cord injury with hemisection at T8 segment was established. The rats were divided into three groups randomly: scaffold group, neural stem cel s group and co-graft group. Rats in the scaffold group were implanted with poly (lactide-co-glycolide) scaffold; those in the neural stem cel s group were implanted with the PLGA scaffolds inoculated with neural stem cel s (labeled with green fluorescence protein); while those in the co-graft group were implanted with PLGA scaffolds inoculated with neural stem cel s (labeled with green fluorescence protein) and Schwann cel s. RESULTS AND CONCLUSION: Transplanted neural stem cel s could survive in the injured spinal cord of rats and migrate near to the spinal cord. Survival rate of positive cel s labeled with green fluorescence protein of the co-graft group was significantly higher than that in the neural stem cel s group (P < 0.001). In the co-graft group, glial fibril ary acidic protein/green fluorescence protein double-positive cel s were more than neuronspecific enolase/green fluorescence protein double-positive cel s. However, no neuronspecific enolase/green fluorescence protein double-positive cel s could be found in the neural stem cel s group. In the co-graft group, only a little part of green fluorescence protein positive cel s expressed synaptophysin. Compared to the other two groups, there was a remarkable increase in the number of regenerated and myelinated axons in the co-graft group. But there was no significant difference in the number of myelinated axons among the three groups (P=0.058). These results suggest that cografted with Schwann cel s, neural stem cel s can be promoted to differentiate into neuron-like cel s, of which smal parts can form synaptic connection. Besides, the poly (lactide-co-glycolide) scaffolds inoculated with neural stem cel s and Schwann cel s can promote axonal regeneration and myelinization.