CAJ | 학술논문

背景:人类胚胎干细胞是来源于着床前囊胚的内细胞团,能在长期培养中无限增殖并保持未分化状态,且具有分化成人体组织各种细胞类型能力的细胞.目的:进一步验证人胚胎干细胞HuES17细胞株向造血干细胞分化的能力.方法:人胚胎干细胞 HuES17采用与人包皮成纤维细胞二维共培养的方式培养,采用人胚胎干细胞与小鼠骨髓基质细胞(OP9)二维共培养的方法诱导胚胎干细胞向造血干细胞分化.结果与结论:人胚胎干细胞与小鼠骨髓基质细胞(OP9)二维共培养诱导造血分化的第四五天即开始出现OP9细胞逐渐老化,很快死亡;可以观察到人胚胎干细胞分化,然而,随着OP9细胞死亡,分化的人胚胎干细胞亦死亡,不能诱导人胚胎干细胞向造血干细胞分化.提示人胚胎干细胞 HuES17细胞株可能不能向造血干细胞分化,或向造血干细胞分化的能力较低.
배경:인류배태간세포시래원우착상전낭배적내세포단,능재장기배양중무한증식병보지미분화상태,차구유분화성인체조직각충세포류형능력적세포.목적:진일보험증인배태간세포HuES17세포주향조혈간세포분화적능력.방법:인배태간세포 HuES17채용여인포피성섬유세포이유공배양적방식배양,채용인배태간세포여소서골수기질세포(OP9)이유공배양적방법유도배태간세포향조혈간세포분화.결과여결론:인배태간세포여소서골수기질세포(OP9)이유공배양유도조혈분화적제사오천즉개시출현OP9세포축점노화,흔쾌사망;가이관찰도인배태간세포분화,연이,수착OP9세포사망,분화적인배태간세포역사망,불능유도인배태간세포향조혈간세포분화.제시인배태간세포 HuES17세포주가능불능향조혈간세포분화,혹향조혈간세포분화적능력교저.
BACKGROUND:Human embryonic stem cel s are derived from inner cel mass of the human blastocyst. The human embryonic stem cel s are capable of the unlimited proliferation and maintain the undifferentiated state in long-term culturing in vitro, and can be differentiated into al kinds of cel types of the human body organizations. OBJECTIVE:To study the hematopoietic differentiation of human embryonic stem cel s HuES17 cel line. METHODS:Human embryonic stem cel s HuES17 were co-cultured with human foreskin fibroblast cel s, and the human embryonic stem cel s were induced to differentiate into hematopoietic stem cel s by co-culturing with bone marrow stromal cel s in mice (OP9 cel s). RESULTS AND CONCLUSION:When human embryonic stem cel s co-cultured with OP9 cel s for 5-6 days, the OP9 cel s began to aging, and soon died;the differentiation of human embryonic stem cel s could be observed. However, with the OP9 cel s death, the differentiated human embryonic stem cel s were also dead, and could not induce the human embryonic stem cel s to differentiate into hematopoietic stem cel s. Human embryonic stem cel s HuES17 cel s line showed little inclination to differentiate into hematopoietic stem cel s, or may not be induced to differentiate into hematopoietic stem cel s.