CAJ | 학술논문

背景:研究报道骨髓间充质干细胞移植可用于治疗急性肾损害,人羊膜间充质干细胞在其他组织器官损伤修复中具有良好的治疗作用.目的:观察人羊膜间充质干细胞移植对顺铂诱导小鼠急性肾损害的治疗作用.方法:分离、培养、鉴定人羊膜间充质干细胞.雌性ICR小鼠随机被分为3组,除空白组外,其余均诱导小鼠急性肾损害模型.人羊膜间充质干细胞移植组第8天尾静脉缓慢注射1.0×1010 L-1浓度的人羊膜间充质干细胞0.3 mL;模型组和空白组尾静脉均注入等量生理盐水.结果与结论:顺铂诱导急性肾损害小鼠人羊膜间充质干细胞移植17 d,生化指标检测结果显示,与模型组比较,人羊膜间充质干细胞移植组小鼠血清肌酐和血尿素氮水平明显降低,且已达空白组水平.肾组织苏木精-伊红染色后,组织病理检查可见人羊膜间充质干细胞移植组小鼠,肾组织结构清晰、完整,肾小管丰富,完整性和结构与空白组相似,较模型组病理学变化改善明显.人羊膜间充质干细胞移植7 d,双色荧光染色可见大量人羊膜间充质干细胞在肾组织中有大量定植和分布,肾小管上皮细胞中也有少量人羊膜间充质干细胞来源的MAB1281-FITC阳性细胞分布.结果表明,人羊膜间充质干细胞移植可促进急性肾损害的修复.
배경:연구보도골수간충질간세포이식가용우치료급성신손해,인양막간충질간세포재기타조직기관손상수복중구유량호적치료작용.목적:관찰인양막간충질간세포이식대순박유도소서급성신손해적치료작용.방법:분리、배양、감정인양막간충질간세포.자성ICR소서수궤피분위3조,제공백조외,기여균유도소서급성신손해모형.인양막간충질간세포이식조제8천미정맥완만주사1.0×1010 L-1농도적인양막간충질간세포0.3 mL;모형조화공백조미정맥균주입등량생리염수.결과여결론:순박유도급성신손해소서인양막간충질간세포이식17 d,생화지표검측결과현시,여모형조비교,인양막간충질간세포이식조소서혈청기항화혈뇨소담수평명현강저,차이체공백조수평.신조직소목정-이홍염색후,조직병리검사가견인양막간충질간세포이식조소서,신조직결구청석、완정,신소관봉부,완정성화결구여공백조상사,교모형조병이학변화개선명현.인양막간충질간세포이식7 d,쌍색형광염색가견대량인양막간충질간세포재신조직중유대량정식화분포,신소관상피세포중야유소량인양막간충질간세포래원적MAB1281-FITC양성세포분포.결과표명,인양막간충질간세포이식가촉진급성신손해적수복.
BACKGROUND:Recent researches have reported that bone marrow mesenchymal stem cel s transplantation can be used for the treatment of acute kidney injury, and human amnion-derived mesenchymal stem cel s have a good therapeutic effect in repairing other tissues and organs injury. OBJECTIVE:To investigate the effect of the human amnion-derived mesenchymal stem cel s transplantation on cisplatin-induced acute kidney injury. METHODS:Human amnion-derived mesenchymal stem cel s were isolated, cultured and identified in vitro. Male ICR mice were randomly divided into three groups:normal control group, model group, human amnion-derived mesenchymal stem cel s group, and mices in the model group and human amnion-derived mesenchymal stem cel s group were used to establish acute kidney injury models. In the human amnion-derived mesenchymal stem cel s group, 0.3 mL human amnion-derived mesenchymal stem cel s with the concentration of 1.0×1010/L were injected into the mice via tail vein at 8 days;mice in the model group and normal control group were injected with normal saline in the same dose. RESULTS AND CONCLUSION:At 17 days after the establishment of cisplatin-induced acute kidney injury model by human amnion-derived mesenchymal stem cel s transplantation, renal function examination results demonstrated that compared with model group, the blood urea nitrogen and serum creatinine levels in human amnion-derived mesenchymal stem cel s group were significantly increased, and reached to the levels in normal control group. Hematoxylin-eosin staining results showed the clear and complete renal organizational structure with rich tubules in the human amnion-derived mesenchymal stem cel s group, the integrity and structure were similar to those of the mice in the normal control group, but the pathological changes of the human amnion-derived mesenchymal stem cel s group were significantly improved when compared with model group. At 7 days after human amnion-derived mesenchymal stem cel s transplantation, double labeling immunofluorescence results showed there was a large number of human amnion-derived mesenchymal stem cel s colonization and distributionin in the nephridial tissue, a few MAB1281-FITC positive cel s derived from human amnion-derived mesenchymal stem cel s also found in the epithelium of kidney tubules. The human amnion-derived mesenchymal stem cel s transplantation could promote the recovery of acute kidney injury.