CAJ | 학술논문

背景:自体骨髓间充质干细胞体外诱导培养后的多方向分化潜能,体内植入能否修复股骨头坏死成为临床骨科的一个研究热点.目的:柚皮甙诱导的骨髓间充质干细胞修复兔股骨头坏死头的作用.方法:柚皮甙诱导兔骨髓间充质干细胞向成骨方向分化,用自制同种异体松质骨载体负载,制备兔股骨头坏死模型,造模后第3周将12只成年新西兰大白兔随机数字表法分为2组,实验组左侧置入空白载体,对照组左侧不植入任何物品;两组右侧均置入复合有诱导后骨髓间充质干细胞的载体.结果与结论:植入后8周植入物表面有大量骨痂形成,与骨端连接紧密,少部形成连续性骨桥.12周部分充填区广布相对较成熟的骨小梁,但较正常骨小梁粗、排列不够规则,可见缺损区完全被新骨代替,提示柚皮甙诱导的骨髓间充质干细胞可修复兔股骨头坏死.
배경:자체골수간충질간세포체외유도배양후적다방향분화잠능,체내식입능부수복고골두배사성위림상골과적일개연구열점.목적:유피대유도적골수간충질간세포수복토고골두배사두적작용.방법:유피대유도토골수간충질간세포향성골방향분화,용자제동충이체송질골재체부재,제비토고골두배사모형,조모후제3주장12지성년신서란대백토수궤수자표법분위2조,실험조좌측치입공백재체,대조조좌측불식입임하물품;량조우측균치입복합유유도후골수간충질간세포적재체.결과여결론:식입후8주식입물표면유대량골가형성,여골단련접긴밀,소부형성련속성골교.12주부분충전구엄포상대교성숙적골소량,단교정상골소량조、배렬불구규칙,가견결손구완전피신골대체,제시유피대유도적골수간충질간세포가수복토고골두배사.
BACKGROUND:Autologous bone marrow mesenchymal stem cel s exhibit multipotential differentiation after in vitro culture. There is increasing concern that whether transplantation of autologous bone marrow mesenchymal stem cel s can be used for repair of femoral head necrosis. OBJECTIVE:To investigate the mechanism by which naringin induces bone marrow mesenchymal stem cel s to repair femoral head necrosis in rabbits. METHODS:Rabbit bone marrow mesenchymal stem cel s were induced with naringin to differentiate into osteoblasts and then loaded onto cancel ous bone al ograft. Femoral head necrosis rabbit models were prepared. At 3 weeks after femoral head necrosis induction, 12 adult New Zealand rabbits were randomly divided into two groups. In the experimental group, blank cancel ous bone al ograft was implanted into the left femoral head necrosis region. Identical y, nothing was implanted into the left femoral head necrosis region in the control group. The right femoral head necrosis region in each group was implanted with cancel ous bone al ograft loaded by naringin induced bone marrow mesenchymal stem cel s. RESULTS AND CONCLUTION:At 8 weeks after implantation, a large amount of cal us formed on the surface of the al ograft and connected tightly with bone ends, and continuous“bone bridge”formed in some parts. At 12 weeks, relatively mature bone trabeculae were observed in the fil ing area, which were thicker, and arranged more irregularly compared with normal bone trabeculae. Bone defect region was completely covered by the newly formed bone. These findings suggest that naringin-induced bone marrow mesenchymal stem cel s can be used for repair of femoral head necrosis in rabbits.