CAJ | 학술논문

背景:科研中常用到单一特定的干细胞,但如何证明所提取的细胞是必需细胞就要用到鉴定技术.目的:介绍分离后的骨髓间充质干细胞和骨髓造血干细胞鉴定技术.方法:由第一作者检索1998年1月至2010年12月PubMed数据及维普数据库有关骨髓间充质干细胞及骨髓造血干细胞的鉴定的文献.英文检索词为“Bone marrow stem cel ,bone marrow hematopoietic stem cel s , Identification”;中文检索词为“骨髓造血干细胞;骨髓间充质干细胞;鉴定”.排除重复性研究,31篇进行归纳总结.结果与结论:目前没有特定的某一种表面标记物能作为鉴定骨髓间充质干细胞的“金标准”,可从3方面对其进行检测:细胞形态和培养特性;细胞标记分子;多种分化潜能.一般认为细胞呈类纤维细胞形态、培养可以贴壁分裂增生具有表面CD44、CD29的细胞,而不表达CD34、CD45等阴性标记物,可初步判断为间充质干细胞.目前真正的造血干细胞表型尚难以确定.造血干细胞的检测主要方法有脾克隆形成法、外克隆形成法及流式细胞测量法等.
배경:과연중상용도단일특정적간세포,단여하증명소제취적세포시필수세포취요용도감정기술.목적:개소분리후적골수간충질간세포화골수조혈간세포감정기술.방법:유제일작자검색1998년1월지2010년12월PubMed수거급유보수거고유관골수간충질간세포급골수조혈간세포적감정적문헌.영문검색사위“Bone marrow stem cel ,bone marrow hematopoietic stem cel s , Identification”;중문검색사위“골수조혈간세포;골수간충질간세포;감정”.배제중복성연구,31편진행귀납총결.결과여결론:목전몰유특정적모일충표면표기물능작위감정골수간충질간세포적“금표준”,가종3방면대기진행검측:세포형태화배양특성;세포표기분자;다충분화잠능.일반인위세포정류섬유세포형태、배양가이첩벽분렬증생구유표면CD44、CD29적세포,이불표체CD34、CD45등음성표기물,가초보판단위간충질간세포.목전진정적조혈간세포표형상난이학정.조혈간세포적검측주요방법유비극륭형성법、외극륭형성법급류식세포측량법등.
BACKGROUND:Single specific stem cel s are always used in the scientific research. An identification technique is required to confirm the isolated cel s. OBJECTIVE:To introduce the identification technique used to confirm the isolated bone marrow mesecnhymal stem cel s and bone marrow hemopoietic stem cel s. METHODS:A computer-based online retrieval was performed to search papers describing identification of bone marrow mesenchymal stem cel s and bone marrow hemopoietic stem cel s published during 1998-01/2010-12 in the PubMed database and Weipu database. The key words are bone marrow stem cel , bone marrow hematopoietic stem cel s, identification in Chinese and English. After excluding repetitive studies, 31 papers were included in the final analysis. RESULTS AND CONCLUSION:There has been no specific surface marker as a gold standard to identify bone marrow mesenchymal stem cel s. Bone marrow mesenchymal stem cel s can be detected from cel morphology and culture feature, molecular marker, multi-differentiation potential. General y, the cel s that have a fibrocyte-like morphology, can be adhered, divided, and proliferated, and express CD44 and CD29 rather than CD34 and CD45, are considered as bone marrow mesenchymal stem cel s. At present, the real phenotype for bone marrow hemopoietic stem cel s has not been determined. Spleen clone formation, in vitro clone formation and flow cytometry are the primarily used methods to detect bone marrow hemopoietic stem cel s.