CAJ | 학술논문

背景:芒果苷以及含芒果苷植物提取物具有多方面生理和药理作用,但其是否促进成骨细胞增殖尚未见报道.目的:验证芒果苷对体外培养SD大鼠成骨细胞增殖的影响. 方法:取新生的SD大鼠的颅骨,二次酶消化法培养成骨细胞.应用MTT法及细胞周期的流式细胞仪检测,观察不同浓度的芒果苷对大鼠成骨细胞毒性,不同浓度的芒果苷对体外培养成骨细胞增殖的影响. 结果与结论:在一定浓度范围内,不同浓度的芒果苷均可促进成骨细胞增殖、分化,并呈现出一定的量效、时效关系.芒果苷最佳促进增殖浓度为40μmol/L.药物作用时间48,72 h,芒果苷20,40,80μmol/L组均有显著促进成骨细胞增殖的作用(P<0.01或P<0.05).芒果苷浓度20,40,80μmol/L等各组细胞S期比值均较正常细胞显著增高,以芒果苷40μmol/L浓度组增高最显著(P <0.05).表明在一定浓度范围内,芒果苷具有促进体外成骨细胞增殖的作用.
배경:망과감이급함망과감식물제취물구유다방면생리화약리작용,단기시부촉진성골세포증식상미견보도.목적:험증망과감대체외배양SD대서성골세포증식적영향. 방법:취신생적SD대서적로골,이차매소화법배양성골세포.응용MTT법급세포주기적류식세포의검측,관찰불동농도적망과감대대서성골세포독성,불동농도적망과감대체외배양성골세포증식적영향. 결과여결론:재일정농도범위내,불동농도적망과감균가촉진성골세포증식、분화,병정현출일정적량효、시효관계.망과감최가촉진증식농도위40μmol/L.약물작용시간48,72 h,망과감20,40,80μmol/L조균유현저촉진성골세포증식적작용(P<0.01혹P<0.05).망과감농도20,40,80μmol/L등각조세포S기비치균교정상세포현저증고,이망과감40μmol/L농도조증고최현저(P <0.05).표명재일정농도범위내,망과감구유촉진체외성골세포증식적작용.
BACKGROUND:Mangiferin and its extracts have many physiological and pharmacological effects, but whether they can promote the proliferation of osteoblasts has not been reported. @@@@OBJECTIVE:To observe the effects of mangiferin on the proliferation of osteoblasts cultured in vitro from SD rats. @@@@METHODS:The osteoblasts from the skul of neonatal SD rats were cultured by the second enzyme digestion. The MTT method and flow cytometry were used to observe the cytotoxicity proliferation of osteoblasts cultured in vitro by different concentrations of mangiferin at different time points. @@@@RESULTS AND CONCLUSION:In a certain range of concentrations, different concentrations of mangiferin could promote the proliferation and differentiation of osteoblasts in a certain dose and time dependent manner. The optimal concentration of mangiferin was 40μmol/L to promote the proliferation of osteoblasts. When the drug action time was 48 and 72 hours, 20, 40 and 80μmol/L mangiferin could significantly promote osteoblast proliferation (P<0.01 or P<0.05). The S-phase fraction of osteoblasts treated with 20, 40, and 80μmol/L mangiferin was significantly increased than that of normal cel s, especial y those treated with 40μmol/L mangiferin (P<0.05). These findings indicate that in a certain range of concentrations, mangiferin can promote the proliferation of osteoblasts in vitro.