中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2012年
50期
9407-9413
,共7页
杨彬%李洲进%邓志%吴官保
楊彬%李洲進%鄧誌%吳官保
양빈%리주진%산지%오관보
独活寄生汤%腰椎间盘突出症%前列腺素E2%扶他林%椎间盘%髓核%纤维环%炎性反应%抗炎%治疗
獨活寄生湯%腰椎間盤突齣癥%前列腺素E2%扶他林%椎間盤%髓覈%纖維環%炎性反應%抗炎%治療
독활기생탕%요추간반돌출증%전렬선소E2%부타림%추간반%수핵%섬유배%염성반응%항염%치료
背景:独活寄生汤是中医药治疗腰椎间盘突出症的基本方,但其具体的作用机制尚不明确.
目的:观察独活寄生汤对腰椎间盘突出症模型兔椎间盘局部组织及血浆前列腺素E2的影响.
方法:采用造模器造模法建立腰椎间盘突出症新西兰家兔模型,于造模后1周行CT检查确定造模成功后,分别给予13.8 g/kg独活寄生汤、2.3 mg/kg扶他林或等量生理盐水进行灌胃,2次/d,连续灌胃2周.并以正常及假手术兔作为对照.
结果与结论:①苏木精-伊红染色显示腰椎间盘突出症模型兔病变髓核及纤维环组织内可见肉芽组织长入、新生血管形成及大量炎性细胞浸润,经灌胃独活寄生汤及扶他林后,可见炎性细胞浸润明显减轻.②E LIS A 结果显示,腰椎间盘突出症模型兔局部软组织及外周血浆中的前列腺素 E2含量明显增加,给予独活寄生汤或扶他林后其含量明显下降(P<0.01),而独活寄生汤组和扶他林组比较差异无显著性意义(P>0.05).说明前列腺素E2在腰椎间盘突出症中起重要作用,能介导体内炎性反应;独活寄生汤能通过调控腰椎间盘突出症模型兔体内前列腺素 E2的表达,从而起到治疗作用,且其在抗炎方面的作用与非类固醇类解热镇痛药扶他林相当.
揹景:獨活寄生湯是中醫藥治療腰椎間盤突齣癥的基本方,但其具體的作用機製尚不明確.
目的:觀察獨活寄生湯對腰椎間盤突齣癥模型兔椎間盤跼部組織及血漿前列腺素E2的影響.
方法:採用造模器造模法建立腰椎間盤突齣癥新西蘭傢兔模型,于造模後1週行CT檢查確定造模成功後,分彆給予13.8 g/kg獨活寄生湯、2.3 mg/kg扶他林或等量生理鹽水進行灌胃,2次/d,連續灌胃2週.併以正常及假手術兔作為對照.
結果與結論:①囌木精-伊紅染色顯示腰椎間盤突齣癥模型兔病變髓覈及纖維環組織內可見肉芽組織長入、新生血管形成及大量炎性細胞浸潤,經灌胃獨活寄生湯及扶他林後,可見炎性細胞浸潤明顯減輕.②E LIS A 結果顯示,腰椎間盤突齣癥模型兔跼部軟組織及外週血漿中的前列腺素 E2含量明顯增加,給予獨活寄生湯或扶他林後其含量明顯下降(P<0.01),而獨活寄生湯組和扶他林組比較差異無顯著性意義(P>0.05).說明前列腺素E2在腰椎間盤突齣癥中起重要作用,能介導體內炎性反應;獨活寄生湯能通過調控腰椎間盤突齣癥模型兔體內前列腺素 E2的錶達,從而起到治療作用,且其在抗炎方麵的作用與非類固醇類解熱鎮痛藥扶他林相噹.
배경:독활기생탕시중의약치료요추간반돌출증적기본방,단기구체적작용궤제상불명학.
목적:관찰독활기생탕대요추간반돌출증모형토추간반국부조직급혈장전렬선소E2적영향.
방법:채용조모기조모법건립요추간반돌출증신서란가토모형,우조모후1주행CT검사학정조모성공후,분별급여13.8 g/kg독활기생탕、2.3 mg/kg부타림혹등량생리염수진행관위,2차/d,련속관위2주.병이정상급가수술토작위대조.
결과여결론:①소목정-이홍염색현시요추간반돌출증모형토병변수핵급섬유배조직내가견육아조직장입、신생혈관형성급대량염성세포침윤,경관위독활기생탕급부타림후,가견염성세포침윤명현감경.②E LIS A 결과현시,요추간반돌출증모형토국부연조직급외주혈장중적전렬선소 E2함량명현증가,급여독활기생탕혹부타림후기함량명현하강(P<0.01),이독활기생탕조화부타림조비교차이무현저성의의(P>0.05).설명전렬선소E2재요추간반돌출증중기중요작용,능개도체내염성반응;독활기생탕능통과조공요추간반돌출증모형토체내전렬선소 E2적표체,종이기도치료작용,차기재항염방면적작용여비류고순류해열진통약부타림상당.
BACKGROUND:Duhuojisheng decoction is a basic prescription of Chinese medicine for treatment of lumbar disc herniation, but its mechanism is yet unclear.
@@@@OBJECTIVE:To study the effect of Duhuojisheng decoction on prostaglandin E2 in rabbits with lumbar disc herniation.
@@@@METHODS:Models of lumbar disc herniation were established in New Zealand rabbits using molding device, and verified successful y through CT examination at 1 week after modeling. The model rabbits were randomly divided into model group (treated with normal saline), voltaren group (treated with 2.3 mg/kg voltaren) and Duhuojisheng decoction group (treated with 2.3 mg/kg Duhuojisheng decoction). The treatment was done twice a day, continued 2 weeks. Normal rabbits and rabbits undergoing sham operation were used as controls.nucleus pulpous and annulus fibrosus tissue visible
@@@@RESULTS AND CONCLUSION:①H em atoxylin-eosin staining results showed that granulation tissue ingrowth, neovascularization and massive infiltration of inflammatory cel s were visible in nucleus pulpous and annulus fibrosus tissue after successful modeling of lumbar disc herniation. However, there was a remarkable relief in inflammatory cel infiltration after intragastric administration of Duhuojisheng decoction and voltaren. immunosorbent assay showed that prostaglandin E2 levels in local soft tissues and peripheral plasma were increased significantly after modeling, and then decreased afte administration of Duhuojisheng decoction and voltaren (P<0.01). However, there was no difference in prostaglandin E2 levels after administration of Duhuojisheng decoction and voltaren (P>0.05). These findings indicate that prostaglandin E2 plays a critical role in lumbar disc herniation, mediating in vivo inflammatory reaction. Duhuojisheng decoction can cure lumbar disc herniation through regulation of prostaglandin E2 expression, and its anti-inflammatory mechanism is similar to nonsteroidal anti-inflammatory drugs, such as voltaren.②The results ofenzym e linked