CAJ | 학술논문

背景:人β-防御素在皮肤的固有免疫中占据重要地位,同时由于其广谱的抗菌活性,从而成为抗感染临床研究中的热点. 目的:观察原代培养人皮肤角质形成细胞在浓度梯度肿瘤坏死因子α的刺激下,其免疫分子β-防御素及 Tol 样受体的表达情况. 方法:取8例30岁以下患者包皮组织,分别原代培养获取角质形成细胞,并采用浓度梯度肿瘤坏死因子α(10,50,100,150,200μg/L)进行刺激,实时荧光定量PCR检测β-防御素1,2,3及Tol 样受体2,4的基因表达水平. 结果与结论:肿瘤坏死因子α对β-防御素1,2,3基因表达均有明显诱导作用,且在150μg/L时达到最大效应(P<0.05),相对于对照组分别出现明显的升高,而在200μg/L时又出现明显降低,呈现出浓度依赖性趋势.肿瘤坏死因子α诱导角质形成细胞后,Tol 样受体2,4的表达规律类似于β-防御素.结果可见肿瘤坏死因子α对体外培养的皮肤角质形成细胞有显著的免疫诱导效应.
배경:인β-방어소재피부적고유면역중점거중요지위,동시유우기엄보적항균활성,종이성위항감염림상연구중적열점. 목적:관찰원대배양인피부각질형성세포재농도제도종류배사인자α적자격하,기면역분자β-방어소급 Tol 양수체적표체정황. 방법:취8례30세이하환자포피조직,분별원대배양획취각질형성세포,병채용농도제도종류배사인자α(10,50,100,150,200μg/L)진행자격,실시형광정량PCR검측β-방어소1,2,3급Tol 양수체2,4적기인표체수평. 결과여결론:종류배사인자α대β-방어소1,2,3기인표체균유명현유도작용,차재150μg/L시체도최대효응(P<0.05),상대우대조조분별출현명현적승고,이재200μg/L시우출현명현강저,정현출농도의뢰성추세.종류배사인자α유도각질형성세포후,Tol 양수체2,4적표체규률유사우β-방어소.결과가견종류배사인자α대체외배양적피부각질형성세포유현저적면역유도효응.
BACKGROUND:Humanβ-defensin plays an important role in the innate immune of the skin. Because of its broad spectrum antibiotic activity, humanβ-defensin has become one of the hotspots in the clinical anti-inflammation research. @@@@OBJECTIVE:To show the inducible expression of humanβ-defensin and Tol-like receptor in human skin keratinocytes by graded concentrations of tumor necrosis factor alpha (TNF-α). @@@@METHODS:Healthy foreskin tissue samples were obtained from eight patients aged less than 30 years undergoing prepucectomy. Cultured primary human skin keratinocytes from the foreskin were stimulated with graded concentrations of TNF-α(10, 50, 100, 150, and 200μg/L). Real-time PCR was performed to quantify mRNA expression ofβ-defensin 1, 2, 3 and Tol-like receptor 2, 4 from the stimulated cel s. @@@@RESULTS AND CONCLUSION:Compared with the control group, the expressions ofβ-defensin 1, 2, 3 and Tol-like receptor 2, 4 in keratinocytes induced by TNF-αwere up-regulated apparently and the strongest inducible effect was at the concentration of 150μg/L (P<0.05) and down-regulated again at the concentration of 200μg/L, presenting a dose-dependent manner. Skin keratinocytes showed a marked inductive capacity to produce humanβ-defensin 1, 2, 3 and Tol-like receptor 2, 4 in response to TNF-α.