CAJ | 학술논문

背景:肺泡Ⅱ型上皮细胞是肺泡上皮组织的干细胞,它的损伤和多种肺部疾病密切相关.有研究显示维甲酸不仅能促进发育期大鼠肺泡的形成,还能促进肺损伤后的修复,但也有研究表明维甲酸在肺气肿模型的治疗中无明显作用. 目的:探索维甲酸能否拮抗丙烯醛对原代培养大鼠肺泡Ⅱ型上皮细胞的损伤作用. 方法:综合Dobbs等的细胞分离方法并加以改良,从普通雄性SD大鼠肺组织中成功分离出较高纯度的肺泡Ⅱ型上皮细胞,并行原代培养.应用MTT法检测丙烯醛对肺泡Ⅱ型上皮细胞活性的影响,流式细胞术检测在丙烯醛及维甲酸作用下肺泡Ⅱ型上皮细胞的细胞周期变化. 结果与结论:MTT检测结果显示丙烯醛作用下大鼠肺泡Ⅱ型上皮细胞生长明显受抑,而且肺泡Ⅱ型上皮细胞活性与药物浓度成剂量效应关系.流式细胞仪检测结果显示丙烯醛作用下G1期细胞增加,维甲酸对丙烯醛的拮抗作用不明显.提示丙烯醛对原代培养的肺泡Ⅱ型上皮细胞具有明显的损伤作用,但维甲酸对丙烯醛的拮抗作用不明显,其作用机制还需进一步深入研究.
배경:폐포Ⅱ형상피세포시폐포상피조직적간세포,타적손상화다충폐부질병밀절상관.유연구현시유갑산불부능촉진발육기대서폐포적형성,환능촉진폐손상후적수복,단야유연구표명유갑산재폐기종모형적치료중무명현작용. 목적:탐색유갑산능부길항병희철대원대배양대서폐포Ⅱ형상피세포적손상작용. 방법:종합Dobbs등적세포분리방법병가이개량,종보통웅성SD대서폐조직중성공분리출교고순도적폐포Ⅱ형상피세포,병행원대배양.응용MTT법검측병희철대폐포Ⅱ형상피세포활성적영향,류식세포술검측재병희철급유갑산작용하폐포Ⅱ형상피세포적세포주기변화. 결과여결론:MTT검측결과현시병희철작용하대서폐포Ⅱ형상피세포생장명현수억,이차폐포Ⅱ형상피세포활성여약물농도성제량효응관계.류식세포의검측결과현시병희철작용하G1기세포증가,유갑산대병희철적길항작용불명현.제시병희철대원대배양적폐포Ⅱ형상피세포구유명현적손상작용,단유갑산대병희철적길항작용불명현,기작용궤제환수진일보심입연구.
BACKGROUND:Alveolar epithelial cel s type Ⅱ AECII (AECⅡ) is the stem cel s of alveolar epithelial tissue, and most of lung diseases are closely related with the damage of AECⅡ. Studies have demonstrated that retinoic acid cannot only promote the rat alveolar formation during the development, but also promote the repair of lung injuries. However, several studies have shown that retinoic acid plays an insignificant role in the treatment of emphysema models. @@@@OBJECTIVE:To explore whether retinoic acid can protect AECⅡ against acrolein. @@@@METHODS:AECⅡ were isolated and purified from the lung tissue of male Sprague-Dawley rats using modified method, fol owed by primary culture. Then, MTT method was done to observe the cel viability of AECⅡ stimulated by acrolein, and cel cycle changes induced by acrolein and/or retinoic acid were analyzed by flow cytometry. @@@@RESULTS AND CONCLUSION:The cel viability fel down by the treatment of acrolein in a dose-dependent manner. The result of flow cytometry showed that cel population in G1 phase increased significantly after treatment with acrolein, but retinoic acid had no antagonistic action to acrolein. The relevant mechanism stil needs further studies.