CAJ | 학술논문

背景:假设支气管和肺成纤维细胞在损伤修复过程中可能表现出不同的生物学特性,从而在慢性阻塞性肺病气道炎症过程中,支气管和肺组织呈现出不同的重构特点. 目的:在体外培养的人肺和支气管成纤维细胞上,观察骨架蛋白的表达以及二者增殖特性的差别,以助阐明慢性阻塞性肺病支气管和肺组织重构的发生机制. 方法:采用人肺和支气管成纤维细胞体外培养;免疫组织化学方法测定细胞波形蛋白和肌动蛋白的表达;MTT法测定细胞增殖. 结果与结论:人肺和支气管成纤维细胞均显著表达波形蛋白和肌动蛋白,但两种蛋白在细胞内的分布不同.波形蛋白呈斑点状分布于肺成纤维细胞胞浆内,并以细胞核周围为主;在支气管成纤维细胞内,波形蛋白均匀分布于细胞浆内.在肺成纤维细胞,肌动蛋白沿细胞膜分布;在支气管成纤维细胞,肌动蛋白广泛分布于细胞浆.在相同的培养条件下,支气管和肺组织成纤维细胞的增殖速度不同,支气管成纤维细胞增殖较肺成纤维细胞增殖速度显著增加.提示支气管和肺成纤维细胞增殖修复特性不同,这些生物学特性的差别可能在慢性阻塞性肺病支气管和肺组织不同的重构过程中发挥重要作用.
배경:가설지기관화폐성섬유세포재손상수복과정중가능표현출불동적생물학특성,종이재만성조새성폐병기도염증과정중,지기관화폐조직정현출불동적중구특점. 목적:재체외배양적인폐화지기관성섬유세포상,관찰골가단백적표체이급이자증식특성적차별,이조천명만성조새성폐병지기관화폐조직중구적발생궤제. 방법:채용인폐화지기관성섬유세포체외배양;면역조직화학방법측정세포파형단백화기동단백적표체;MTT법측정세포증식. 결과여결론:인폐화지기관성섬유세포균현저표체파형단백화기동단백,단량충단백재세포내적분포불동.파형단백정반점상분포우폐성섬유세포포장내,병이세포핵주위위주;재지기관성섬유세포내,파형단백균균분포우세포장내.재폐성섬유세포,기동단백연세포막분포;재지기관성섬유세포,기동단백엄범분포우세포장.재상동적배양조건하,지기관화폐조직성섬유세포적증식속도불동,지기관성섬유세포증식교폐성섬유세포증식속도현저증가.제시지기관화폐성섬유세포증식수복특성불동,저사생물학특성적차별가능재만성조새성폐병지기관화폐조직불동적중구과정중발휘중요작용.
BACKGROUND:The bronchial and lung tissue shows different reconstruction characteristics in the process of chronic obstructive pulmonary disease as the bronchial and lung fibroblasts may exhibit different biological characteristics during the process for repairing defects. @@@@OBJECTIVE:To observe the cytoskeleton protein expression and the proliferation of human lung and bronchial fibroblasts cultured in vitro in order to clarify the mechanism of bronchial and lung tissue remodeling of chronic obstructive pulmonary disease. @@@@METHODS:Human lung and bronchial fibroblasts were cultured in vitro;the expression of vimentin andαsmooth muscle actin was detected by immunohistochemistry;MTT assay was utilized for measuring the fibroblasts proliferation. @@@@RESULTS AND CONCLUSION:Vimentin andαsmooth muscle actin were intensely expressed in the human lung and bronchial fibroblasts, but the distribution was different between lung and bronchial fibroblasts. In the cytoplasm of lung fibroblasts, vimentin was expressed mainly around the nuclear in a dot-like pattern, and vimentin was distributed along the cel membrane. In the bronchial fibroblasts, the vimentin was distributed along the cel membrane;in the lung fibroblasts,αsmooth muscle actin was distributed in the cel membrane. Under the same culture condition, the proliferative degree of lung and bronchial fibroblasts was different. The bronchial fibroblasts proliferated significantly faster than the lung fibroblasts. These findings suggest that bronchial and lung fibroblasts behave differently in the repair and regenerative process of lung and bronchial tissue remodeling, which might play a pivotal role in the airflow limitation of chronic obstructive pulmonary disease.