CAJ | 학술논문

背景:机械应力在软骨组织工程中起着十分重要的作用,但应力对异体种子细胞诱导分化的作用研究较少,应力促进异体干细胞向软骨细胞分化的机制尚不清楚. 目的:探讨降低干细胞的免疫原性以最终将异体种子细胞构建的组织工程化软骨应用于临床的可能性. 方法:应用计算机检索2000年1月至2012年1月 PubMed 数据库相关文章,检索词为“engineered articular cartilage tissue,cartilage immune,articular cartilage,biomechanical”.共检索到文献510篇,最终纳入符合标准的文献33篇. 结果与结论:利用干细胞多向分化潜能将其诱导分化为成软骨细胞,研究力学载荷对软骨细胞的影响、力学信号的传导机制以便应用生物反应器将软骨细胞进行大规模扩增;利用基因打靶技术可使体外培养的干细胞染色体DNA与外源性同源序列发生重组,达到定点修饰改造细胞β2-微球蛋白基因的目的,降低干细胞主要组织相容性复合体Ⅰ类分子的表达,从而降低其免疫原性,为软骨组织工程提供低免疫原性的种子细胞.
배경:궤계응력재연골조직공정중기착십분중요적작용,단응력대이체충자세포유도분화적작용연구교소,응력촉진이체간세포향연골세포분화적궤제상불청초. 목적:탐토강저간세포적면역원성이최종장이체충자세포구건적조직공정화연골응용우림상적가능성. 방법:응용계산궤검색2000년1월지2012년1월 PubMed 수거고상관문장,검색사위“engineered articular cartilage tissue,cartilage immune,articular cartilage,biomechanical”.공검색도문헌510편,최종납입부합표준적문헌33편. 결과여결론:이용간세포다향분화잠능장기유도분화위성연골세포,연구역학재하대연골세포적영향、역학신호적전도궤제이편응용생물반응기장연골세포진행대규모확증;이용기인타파기술가사체외배양적간세포염색체DNA여외원성동원서렬발생중조,체도정점수식개조세포β2-미구단백기인적목적,강저간세포주요조직상용성복합체Ⅰ류분자적표체,종이강저기면역원성,위연골조직공정제공저면역원성적충자세포.
BACKGROUND:Mechanical stress plays a very important role in cartilage tissue engineering, but there are fewer studies on stress induced al ogeneic seed cel differentiation and the mechanism of stress to promote differentiation of al ogeneic stem cel s into chondrocytes is stil unclear. @@@@OBJECTIVE:To explore the feasibility of reducing immunogenicity of stem cel s to apply al ogeneic seed cel s in the construction of tissue-engineered cartilage in clinic. @@@@METHODS:A computer-based search of PubMed (2000-01/2012-01) was performed using the keywords of“engineered articular cartilage tissue, cartilage immune, articular cartilage, biomechanical”. The language was limited to English. Total y 510 articles were retrieved, and final y 33 articles were included in result analysis. @@@@RESULTS AND CONCLUSION:Stem cel s with multilineage differentiation potential differentiate into chondrocytes. Mechanical load effects on chondrocytes and mechanical signal transduction mechanism have been explored to carry out a large-scale proliferation of chondrocytes using bioreactor. Gene targeting technology enables the recombination of chromosomal DNA from stem cel s cultured in vitro and exogenous homologous sequence to modifyβ2-microglobulin gene, reduce the immunogenicity of stem cel s, and provide low-immunogenicity seed cel s for cartilage tissue engineering.