CAJ | 학술논문

背景:国内关于纳米粒子在水及磷酸盐缓冲液中稳定性的研究报道较少. 目的:考察Pluronic F127/聚乳酸纳米粒子在水及磷酸盐缓冲液中的稳定性及Pluronic F127/聚乳酸聚合物作为药物载体的可行性. 方法:采用透析法制备Pluronic F127/聚乳酸纳米粒子,通过高效液相色谱研究该纳米粒子作为药物载体包埋紫杉醇及体外释放行为,同时采用MTT法考察该聚合物的细胞毒性. 结果与结论:Pluronic F127/聚乳酸纳米粒子在水中的稳定性优于磷酸盐缓冲液,其次,温度对于纳米粒子的稳定性影响较大,无论是磷酸盐缓冲液还是水中,37℃条件下粒径变化均较大,因此纳米粒子在低温下稳定性较好.包埋紫杉醇的Pluronic F127/聚乳酸纳米粒子的释放曲线在前20 h内呈现快速释放,此后表现为缓慢释放,约有20%的紫杉醇释放出来,MTT测试结果表明Pluronic F127/聚乳酸嵌段共聚物具有很好的生物相容性.综合以上结果表明, Pluronic F127/聚乳酸适合用作药物载体.
배경:국내관우납미입자재수급린산염완충액중은정성적연구보도교소. 목적:고찰Pluronic F127/취유산납미입자재수급린산염완충액중적은정성급Pluronic F127/취유산취합물작위약물재체적가행성. 방법:채용투석법제비Pluronic F127/취유산납미입자,통과고효액상색보연구해납미입자작위약물재체포매자삼순급체외석방행위,동시채용MTT법고찰해취합물적세포독성. 결과여결론:Pluronic F127/취유산납미입자재수중적은정성우우린산염완충액,기차,온도대우납미입자적은정성영향교대,무론시린산염완충액환시수중,37℃조건하립경변화균교대,인차납미입자재저온하은정성교호.포매자삼순적Pluronic F127/취유산납미입자적석방곡선재전20 h내정현쾌속석방,차후표현위완만석방,약유20%적자삼순석방출래,MTT측시결과표명Pluronic F127/취유산감단공취물구유흔호적생물상용성.종합이상결과표명, Pluronic F127/취유산괄합용작약물재체.
BACKGROUND:Few reports about the stability of nanoparticles in the water and PBS have been published in China. @@@@OBJECTIVE:To investigate the stability of pluronic/poly(lactic acid) nanoparcticles in the water and PBS and feasibility of pluronic F127/poly(lactic acid) block polymer as drug carriers @@@@METHODS:Pluronic poly(lactic acid) nanoparcticles were prepared by a dialysis method. In vitro high performance liquid chromatography, and the cytotoxicity of pluronic F127/poly(lactic acid) block polymer was investigated by MTT. @@@@RESULTS AND CONCLUSION:Pluronic/poly(lactic acid) nanoparticles had better stability in the water than in the PBS. In addition, pluronic/poly(lactic acid) nanoparticles were influenced greatly by the temperature, which had a good stability in low temperature. At 37 ℃, pluronic/poly(lactic acid) nanoparticles had a greater change in the particle size. The release curve of paclitaxel-loaded pluronic F127/poly(lactic acid) nanoparticles showed a rapid release within 20 hours, and then presented with a slow release. Approximately 20%paclitaxel was released from the nanoparticles. MTT test results revealed that pluronic F127/poly(lactic acid) block polymer had a good biocompatibility. These findings indicate that pluronic/poly(lactic acid) nanoparticles can be used as drug carriers.