CAJ | 학술논문

背景:骨形态发生蛋白在骨组织工程中很易降解,聚乙烯吡咯啉酮理论上存在与骨形态发生蛋白结合并对其缓释的作用. 目的:观察聚乙稀吡咯啉酮修饰的纳米晶胶原基骨复合骨形态发生蛋白的体外缓释效果,并与成骨细胞复合培养,以期得到一种生物相容性较为理想的缓释支架系统. 方法:实验分为3组,即聚乙烯吡咯啉酮修饰的骨形态发生蛋白复合纳米晶胶原基骨组、骨形态发生蛋白复合纳米晶胶原基骨组及聚乙烯吡咯啉酮修饰的骨形态发生蛋白复合大鼠松质骨组.以ELISA法检测3种复合物的骨形态发生蛋白体外释放活性.将SD大鼠骨髓间充质干细胞分离后定向诱导为成骨细胞并种于支架上,3,7,10,14 d时检测支架中的细胞计数;细胞培养14 d后采用扫描电镜观察细胞生长情况. 结果与结论:骨形态发生蛋白体外释放曲线可见14 d后实验组上清液中骨形态发生蛋白仍保持较高的浓度.培养14 d后,大鼠骨髓间充质干细胞Ⅰ型胶原免疫荧光染色阳性,支架中脱落细胞计数均高于对照组(P<0.05).扫描电镜结果发现实验组支架上的细胞生长情况明显好于对照组.提示聚乙烯吡咯烷酮修饰的纳米晶胶原基骨支架具有良好的缓释骨形态发生蛋白的作用,与大鼠骨髓间充质干细胞有很好的生物相容性.
배경:골형태발생단백재골조직공정중흔역강해,취을희필각람동이론상존재여골형태발생단백결합병대기완석적작용. 목적:관찰취을희필각람동수식적납미정효원기골복합골형태발생단백적체외완석효과,병여성골세포복합배양,이기득도일충생물상용성교위이상적완석지가계통. 방법:실험분위3조,즉취을희필각람동수식적골형태발생단백복합납미정효원기골조、골형태발생단백복합납미정효원기골조급취을희필각람동수식적골형태발생단백복합대서송질골조.이ELISA법검측3충복합물적골형태발생단백체외석방활성.장SD대서골수간충질간세포분리후정향유도위성골세포병충우지가상,3,7,10,14 d시검측지가중적세포계수;세포배양14 d후채용소묘전경관찰세포생장정황. 결과여결론:골형태발생단백체외석방곡선가견14 d후실험조상청액중골형태발생단백잉보지교고적농도.배양14 d후,대서골수간충질간세포Ⅰ형효원면역형광염색양성,지가중탈락세포계수균고우대조조(P<0.05).소묘전경결과발현실험조지가상적세포생장정황명현호우대조조.제시취을희필각완동수식적납미정효원기골지가구유량호적완석골형태발생단백적작용,여대서골수간충질간세포유흔호적생물상용성.
BACKGROUND:Bone morphogenetic protein (BMP) is important in bone tissue engineering and it is easily degraded by protainases in the body. According to the related researches, polyethlenepyrolindone (PVP) can effectively make BMP to releases lower and has good biocompatibility with the stent. @@@@OBJECTIVE:To study the effect of the delayed release system of PVP modified nano-hydroxyapatite, then to cultivate it with bone marrow mesenchymal stem cel s (BMSCs) that have been induced to osteoblasts to prepare a new delayed release system with good biocompatibility. @@@@METHODS:The samples were divided into three groups:PVP modified BMP combined with nano-hydroxyapatite;unmodified BMP combined with nano-hydroxyapatite;PVP modified BMP combined with spongy bone from SD rat bone marrows. First, the activity of PVP-BMP microsphere was detected by ELISA. Second, after the BMSCs were induced and proliferated to osteoblasts, they were seeded onto the scaffold. The number of cel s on the scaffold was counted at different time points (3, 7, 10, 14 days). The cel growth on scaffold was also observed by scanning electron microscope 14 days later. @@@@RESULTS AND CONCLUSION:The expression of BMP was stil higher after 14 days in the experimental group. The differentiation of BMSCs to osteoblastic phenotype was demonstrated by the positive staining of col agen type Ⅰ. The cast-of cel s from the scaffold in the experimental group were obviously more than those in the control group (P<0.05). Scanning electron microscope observed that the cel grew better in the experimental group than in the control group. PVP-modified BMP-nanohydroxyapatite delayed release system has good ef ect, it can cause BMSCs to proliferate and dif erentiate wel .