CAJ | 학술논문

背景:羧甲基壳聚糖或血小板衍生生长因子均可促进对体外培养人牙周膜细胞的增殖. 目的:观察羧甲基壳聚糖和血小板衍生生长因子BB联合应用对体外培养人牙周膜细胞增殖和分化能力的影响. 方法:取生长良好的第4或5代人牙周膜细胞,分组培养:对照组(仅含体积分数2%FBS的DMEM培养液)、10μg/L血小板衍生生长因子BB组、100 mg/L羧甲基壳聚糖+10μg/L 血小板衍生生长因子BB组、800 mg/L 羧甲基壳聚糖+10μg/L 血小板衍生生长因子BB组、100 mg/L羧甲基壳聚糖组、800 mg/L羧甲基壳聚糖组. 结果与结论:①M TT 检测:与对照组比较,其余各组均能促进人牙周膜细胞增殖,且100 mg/L羧甲基壳聚糖+10μg/L血小板衍生生长因子BB、800 mg/L 羧甲基壳聚糖+10μg/L血小板衍生生长因子BB组细胞增殖高于其他组(P <0.05),100 mg/L 羧甲基壳聚糖+10μg/L血小板生性生长因子BB促增殖作用最显著(P<0.05).②细胞周期检测:与MTT检测结果相符.③碱性磷酸酶活性:与对照组比较,除10μg/L 血小板衍生生长因子BB组降低外,其余组均增强(P<0.05).表明羧甲基壳聚糖和血小板衍生生长因子BB联合应用可促进人牙周膜细胞增殖和骨向分化.
배경:최갑기각취당혹혈소판연생생장인자균가촉진대체외배양인아주막세포적증식. 목적:관찰최갑기각취당화혈소판연생생장인자BB연합응용대체외배양인아주막세포증식화분화능력적영향. 방법:취생장량호적제4혹5대인아주막세포,분조배양:대조조(부함체적분수2%FBS적DMEM배양액)、10μg/L혈소판연생생장인자BB조、100 mg/L최갑기각취당+10μg/L 혈소판연생생장인자BB조、800 mg/L 최갑기각취당+10μg/L 혈소판연생생장인자BB조、100 mg/L최갑기각취당조、800 mg/L최갑기각취당조. 결과여결론:①M TT 검측:여대조조비교,기여각조균능촉진인아주막세포증식,차100 mg/L최갑기각취당+10μg/L혈소판연생생장인자BB、800 mg/L 최갑기각취당+10μg/L혈소판연생생장인자BB조세포증식고우기타조(P <0.05),100 mg/L 최갑기각취당+10μg/L혈소판생성생장인자BB촉증식작용최현저(P<0.05).②세포주기검측:여MTT검측결과상부.③감성린산매활성:여대조조비교,제10μg/L 혈소판연생생장인자BB조강저외,기여조균증강(P<0.05).표명최갑기각취당화혈소판연생생장인자BB연합응용가촉진인아주막세포증식화골향분화.
BACKGROUND:Both carboxymethyl chitosan and platelet-derived growth factor-BB can promote the proliferation of human periodontal ligament cel s cultured in vitro. @@@@OBJECTIVE:To explore the effects of carboxymethyl chitosan and platelet-derived growth factor-BB combination on the proliferation and differentiation of human periodontal ligament cel s cultured in vitro. @@@@METHODS:The fourth and the fifth generation of human periodontal ligament cel s were col ected and primarily cultured in different groups:control group (Dulbecco’s modified Eagle’s medium contained 2%fetal bovine serum), 10μg/L platelet-derived growth factor-BB group, 100 mg/L carboxymethyl chitosan+10μg/L platelet-derived growth factor-BB group, 800 mg/L carboxymethyl chitosan group+10μg/L platelet-derived growth factor-BB group, 100 mg/L carboxymethyl chitosan group and 800 mg/L carboxymethyl chitosan group. @@@@RESULTS AND CONCLUSION:(1) Compared with control group, each experimental group can promote the proliferation ability of human periodontal ligament cel s by MTT assay, and the proliferation of the cel s in the combination groups was higher than that in the other groups (P<0.05), and the dose of 100 mg/L carboxymethyl chitosan plus 10μg/L platelet-derived growth factor-BB had the greatest promotion effect on the proliferation of human periodontal ligament cel s (P<0.05). (2) The cel cycle results were in line with the results of the MTT assay. (3) Alkaline phosphatase activity detection:compared with the control group, the alkaline phosphatase activity was increased in al groups except for 10μg/L platelet-derived growth factor-BB group (P<0.05). It indicates that the combination of carboxymethyl chitosan and platelet-derived growth factor-BB can promote the proliferation and osteogenic differentiation of the human periodontal ligament cel s.