CAJ | 학술논문

背景:在微胶囊微环境中肿瘤细胞表现出更接近在体肿瘤的特性. 目的:考察微囊化培养对肿瘤细胞耐药性的影响. 方法:将平面培养至对数生长期的HepG2细胞微囊化培养15 d,再次包封于微胶囊内进行微囊化培养,如此反复3次.回收每次微囊化培养的细胞,并通过显微镜、流式细胞仪、CCK-8和Real Time PCR检测细胞形态、黏附能力、增殖能力、细胞周期、药物敏感性和耐药相关基因的变化. 结果与结论:微囊化培养不同次数的细胞再次进行平面培养,其形态、黏附能力、增殖能力和细胞周期均无显著变化.经过微囊化培养后再次进行平面培养,肿瘤细胞的耐药性随着微囊化培养次数的增加而逐渐下降,且耐药性降低的主要原因是耐药相关基因表达下调.提示只有在微胶囊这一独特微环境中肿瘤细胞才能保持高的耐药性,一旦回归平面培养,这一特性便会消失,肿瘤细胞只有在微囊化环境中培养才能表现出类似在体的耐药性.
배경:재미효낭미배경중종류세포표현출경접근재체종류적특성. 목적:고찰미낭화배양대종류세포내약성적영향. 방법:장평면배양지대수생장기적HepG2세포미낭화배양15 d,재차포봉우미효낭내진행미낭화배양,여차반복3차.회수매차미낭화배양적세포,병통과현미경、류식세포의、CCK-8화Real Time PCR검측세포형태、점부능력、증식능력、세포주기、약물민감성화내약상관기인적변화. 결과여결론:미낭화배양불동차수적세포재차진행평면배양,기형태、점부능력、증식능력화세포주기균무현저변화.경과미낭화배양후재차진행평면배양,종류세포적내약성수착미낭화배양차수적증가이축점하강,차내약성강저적주요원인시내약상관기인표체하조.제시지유재미효낭저일독특미배경중종류세포재능보지고적내약성,일단회귀평면배양,저일특성편회소실,종류세포지유재미낭화배경중배양재능표현출유사재체적내약성.
BACKGROUND:Tumor cel s within the microenvironment created by microcapsules have similar the characteristics of tumors in vivo. @@@@OBJECTIVE:To investigate the influence of microencapsulated culture on drug resistance of tumor cel s. @@@@METHODS:HepG2 cel s obtained from monolayer culture in the exponential growth phase were microencapsulated cultured for 15 days. Again the cel s were encapsulated in microcapsules for the microencapsulated culture. The procedure above was repeated for three times. The cel s after microencapsulated culture were retrieved for further examination. The morphology, adhesion ability, proliferation ability, cel cycle, drug sensitivity and the expression of drug resistance-associated genes were detected through microscope, flow cytometry, Cel Counting Kit-8 assay and real-time PCR, respectively. @@@@RESULTS AND CONCLUSION:The HepG2 cel s went through different times of microencapsulated culture showed no significant changes upon their morphology, adhesion ability, proliferation ability and cel cycle when they were grown in monolayer culture again. Compared with the cel s without microencapsulated culture, the drugs resistance of the retrieved cel s from microcapsules decreased along with the increasing of the microencapsulated culturing times. It was primarily due to the expression of drug resistance-associated genes declined. The results of this study suggested only cultured in the microcapsules, the tumor cel s could maintain the higher drug resistance, and once back to the monolayer culture, the characteristics would disappear. Therefore, the tumor cel s could acquire the in vivo-like drug resistance only when cultured in the microcapsules.