CAJ | 학술논문

背景:前期研究表明羟丁基几丁糖具有良好的生物相容性及极低的细胞毒性,同时具有良好的生物降解性. 目的:探讨羟丁基几丁糖凝胶防止术后脑脊液漏的可行性. 方法:首先利用流变仪和动态力学实验仪检测1.5%,2.0%,3.0%温敏性羟丁基几丁糖凝胶的力学强度及耐疲劳性质,同时测定各浓度温敏性羟丁基几丁糖的凝胶时间.将10只猕猴随机分为实验组与对照组,均制作脑脊液渗漏模型,实验组采用1.5%温敏性羟丁基几丁糖凝胶封堵,对照组未采用任何材料封堵. 结果与结论:1.5%温敏性羟丁基几丁糖的凝胶时间为(70±4) s,承受的最大压力为50.3-60.1 kPa,并且在疲劳实验和频率扫描实验中呈现出良好的稳定性;2.0%温敏性羟丁基几丁糖凝胶的凝胶时间为(45±3) s,承受的最大压力为70.6-122.5 kPa,在频率扫描中呈现了较好的稳定性;3.0%温敏性羟丁基几丁糖凝胶的凝胶时间为(26±2) s,承受的最大压力为77.8-104.7 kPa,频率扫描呈现了较好的稳定性.但后两者的疲劳实验未达实验要求.脑脊液渗漏封堵实验中,实验组封堵率为100%,对照组无封堵效果,表明1.5%温敏性羟丁基几丁糖凝胶可防止猕猴脑脊液漏.
배경:전기연구표명간정기궤정당구유량호적생물상용성급겁저적세포독성,동시구유량호적생물강해성. 목적:탐토간정기궤정당응효방지술후뇌척액루적가행성. 방법:수선이용류변의화동태역학실험의검측1.5%,2.0%,3.0%온민성간정기궤정당응효적역학강도급내피로성질,동시측정각농도온민성간정기궤정당적응효시간.장10지미후수궤분위실험조여대조조,균제작뇌척액삼루모형,실험조채용1.5%온민성간정기궤정당응효봉도,대조조미채용임하재료봉도. 결과여결론:1.5%온민성간정기궤정당적응효시간위(70±4) s,승수적최대압력위50.3-60.1 kPa,병차재피로실험화빈솔소묘실험중정현출량호적은정성;2.0%온민성간정기궤정당응효적응효시간위(45±3) s,승수적최대압력위70.6-122.5 kPa,재빈솔소묘중정현료교호적은정성;3.0%온민성간정기궤정당응효적응효시간위(26±2) s,승수적최대압력위77.8-104.7 kPa,빈솔소묘정현료교호적은정성.단후량자적피로실험미체실험요구.뇌척액삼루봉도실험중,실험조봉도솔위100%,대조조무봉도효과,표명1.5%온민성간정기궤정당응효가방지미후뇌척액루.
BACKGROUND:Previous studies have showed that temperature-responsive hydroxybutyl chitosan has a good biocompatibility, extremely low toxicity, and good biodegradability. @@@@OBJECTIVE:To evaluate the efficacy of a novel biomaterial, temperature-responsive hydroxybutyl chitosan, in the prevention of postoperative cerebrospinal fluid leakage. @@@@METHODS:The basic mechanical properties of temperature-responsive hydroxybutyl chitosan at 1.5%, 2.0%, 3.0%were measured by rheometer and dynamic mechanical instrument, including gelation time, mechanical strength, and anti-fatigue property. Ten macaques were selected to prepare animal models of cerebrospinal fluid leakage, and then randomly divided into experimental and control groups. Temperature-responsive hydroxybutyl chitosan at 1.5%was used in the experimental group and nothing in the control group. @@@@RESULTS AND CONCLUSION:The gelation time of temperature-responsive hydroxybutyl chitosan at 1.5%was (70±4) seconds, and the max sustainable pressure was 50.3-60.1 kPa, which showed excel ent stability in the fatigue tests and frequency scanning. The gelation time of samples at 2%was (45±3) seconds, and the max sustainable pressure was 70.6-122.5 kPa, which failed to pass the fatigue test, but showed excel ent stability in frequency scanning. The gelation time of temperature-responsive hydroxybutyl chitosan at 3%was (26±2) seconds, and the max sustainable pressure was 77.8-104.7 kPa. Likewise, the temperature-responsive hydroxybutyl chitosan at 3%with excel ent stability in frequency scanning also failed to pass the fatigue test. The plugging rate was 100%in the experimental group, but 0 in the control group. Therefore, the temperature-responsive hydroxybutyl chitosan at 1.5%is most suitable for clinical application that confirmed by the animal studies of macaques in prevention of cerebrospinal fluid leakage.