CAJ | 학술논문

背景:丝素蛋白材料具有良好的生物相容性. 目的:观察丝素蛋白材料复合骨髓间充质干细胞修复大鼠全层皮肤缺损的可行性. 方法:应用5-溴脱氧尿核苷标记SD大鼠骨髓间充质干细胞,并复合丝素蛋白材料培养.建立SD大鼠全层皮肤缺损模型,随机分组:实验组移植同种异体骨髓间充质干细胞与丝素蛋白材料复合物,细胞组移植骨髓间充质干细胞,对照组移植丝素蛋白材料,空白组不作处理. 结果与结论:①大体观察:空白组术后8周仍可见坏死组织,且瘢痕挛缩明显;细胞组与空白组愈合情况相近.对照组术后8周未愈合,有少量瘢痕形成,与周围皮肤融为一体.实验组术后4周创面无明显瘢痕形成,术后8周愈合良好.②组织学观察:实验组术后4周免疫荧光染色显示带有5-溴脱氧尿核苷标记的骨髓间充质干细胞定位在重建的表皮和真皮组织中,术后8周创面愈合良好;细胞组免疫荧光染色显示少量带有5-溴脱氧尿核苷标记的骨髓间充质干细胞存在.表明丝素蛋白材料与骨髓间充质干细胞联合移植可修复大鼠全层皮肤缺损.
배경:사소단백재료구유량호적생물상용성. 목적:관찰사소단백재료복합골수간충질간세포수복대서전층피부결손적가행성. 방법:응용5-추탈양뇨핵감표기SD대서골수간충질간세포,병복합사소단백재료배양.건립SD대서전층피부결손모형,수궤분조:실험조이식동충이체골수간충질간세포여사소단백재료복합물,세포조이식골수간충질간세포,대조조이식사소단백재료,공백조불작처리. 결과여결론:①대체관찰:공백조술후8주잉가견배사조직,차반흔련축명현;세포조여공백조유합정황상근.대조조술후8주미유합,유소량반흔형성,여주위피부융위일체.실험조술후4주창면무명현반흔형성,술후8주유합량호.②조직학관찰:실험조술후4주면역형광염색현시대유5-추탈양뇨핵감표기적골수간충질간세포정위재중건적표피화진피조직중,술후8주창면유합량호;세포조면역형광염색현시소량대유5-추탈양뇨핵감표기적골수간충질간세포존재.표명사소단백재료여골수간충질간세포연합이식가수복대서전층피부결손.
BACKGROUND:The silk fibroin material has good biocompatibility. @@@@OBJECTIVE:To observe the feasibility of silk fibroin material and bone marrow mesenchymal stem cel s for repairing the rat ful-thickness cutaneous defects. @@@@METHODS:The bone marrow mesenchymal stem cel s of Sprague-Dawley rats were labeled with 5-bromodeoxyuridine and cultured with silk fibroin material. The Sprague-Dawley rat ful-thickness cutaneous defect models were prepared and divided into four groups:the experimental group was transplanted with the complex of al ogeneic bone marrow mesenchymal stem cel s and silk fibroin material;the cel group was transplanted with bone marrow mesenchymal stem cel s;the control group was transplanted with silk fibroin material;and the blank group without any treatment. @@@@RESULTS AND CONCLUSION: ①General observation:the necrotic tissue was observed in the blank group at 8 weeks after operation and the scar contracture was obvious;the wound healing in the cel group was similar with that in the blank group. The wound in the control group was not healed and a little amount of scar was observed and integrated with the surrounding skin. There was no obvious scar in the experimental group at 4 weeks after operation and the wound was healed wel at 8 weeks after operation. ②Histological observation:the immunofluorescence staining in the experimental group at 4 weeks after operation showed that the 5-bromodeoxyuridine labeled bone marrow mesenchymal stem cel s were located in reconstructed epidermis and dermal tissues, and wound surface was recovered wel at 8 weeks after operation;the immunofluorescence staining in the cel group showed a little amount of 5-bromodeoxyuridine labeled bone marrow mesenchymal stem cel s. It indicates that co-transplantation of silk fibroin material and bone marrow mesenchymal stem cel s can repair rat ful-thickness cutaneous defects.