CAJ | 학술논문

背景:活血通络法是临床防治激素性股骨头坏死的有效治法,活血通络方促进骨坏死组织修复有确切疗效. 目的:观察活血通络法对激素性股骨头坏死家兔模型组织形态学和胰岛素样生长因子1的影响. 方法:家兔随机分为5组,其中4组用糖皮质激素造成激素性股骨头坏死模型,活血通络方高、中、低剂量组分别灌服活血通络汤混悬液10.0,5.0,2.5 mL/kg,模型组灌服10 mL/kg蒸馏水;空白对照组不造模仅灌服10 mL/kg蒸馏水,连续8周. 结果与结论:组织形态学检查显示,模型组骨坏死发生严重,活血通络方各剂量组骨坏死发生轻.空骨陷窝率模型组较空白对照组明显升高(P<0.01),活血通络方各剂量组较模型组明显降低(P<0.05).胰岛素样生长因子1水平模型组较空白对照组升高(P <0.05),活血通络方各剂量组均较模型组明显升高(P <0.01),但活血通络方中高剂量组之间差异无显著性意义(P>0.05).提示活血通络方可能通过升高胰岛素样生长因子1水平来促进坏死股骨头的修复与重建,但当剂量达到一定程度时胰岛素样生长因子1并不随剂量的增大而继续升高.
배경:활혈통락법시림상방치격소성고골두배사적유효치법,활혈통락방촉진골배사조직수복유학절료효. 목적:관찰활혈통락법대격소성고골두배사가토모형조직형태학화이도소양생장인자1적영향. 방법:가토수궤분위5조,기중4조용당피질격소조성격소성고골두배사모형,활혈통락방고、중、저제량조분별관복활혈통락탕혼현액10.0,5.0,2.5 mL/kg,모형조관복10 mL/kg증류수;공백대조조불조모부관복10 mL/kg증류수,련속8주. 결과여결론:조직형태학검사현시,모형조골배사발생엄중,활혈통락방각제량조골배사발생경.공골함와솔모형조교공백대조조명현승고(P<0.01),활혈통락방각제량조교모형조명현강저(P<0.05).이도소양생장인자1수평모형조교공백대조조승고(P <0.05),활혈통락방각제량조균교모형조명현승고(P <0.01),단활혈통락방중고제량조지간차이무현저성의의(P>0.05).제시활혈통락방가능통과승고이도소양생장인자1수평래촉진배사고골두적수복여중건,단당제량체도일정정도시이도소양생장인자1병불수제량적증대이계속승고.
BACKGROUND:Huoxuetongluo method is an effective prescription for the treatment of steroid induced femoral head necrosis in clinic, and Huoxuetongluo can promote osteonecrosis tissue repair. @@@@OBJECTIVE:To investigate the effect of Huoxuetongluo method on the level of insulin-like growth factor-1 in the steroid induced femoral head necrosis rabbit model. @@@@METHODS:The rabbits were randomly divided into five groups, and the rabbits in four groups were used to establish the steroid induced femoral head necrosis model through injecting the glucocorticoid. The rabbits in the high, moderate and low dose groups were administered intramuscularly with 10.0, 5.0 and 2.5 mL/kg Huoxuetongluo decoction, rabbits in both the model group and the blank control group were administered intramuscularly with 10 mL/kg normal saline and lasted for 8 weeks. @@@@RESULTS AND CONCLUSION:Histomorphometric examination showed that the femoral head necrosis in the model group was severe, while the femoral head necrosis in the high, moderate and low dose groups was mild necrosis. The data of empty lacuna in the model group was significantly higher than that in the blank control group (P<0.01), and the level in the high, moderate and low dose groups was lower than that in the model group (P<0.05);the level of insulin-like growth factor-1 in the model group was higher than the in the blank control group (P<0.05) and the level in the high, moderate and low dose groups was higher than that in the model group (P<0.01), while there was no significant difference between the every two Huoxuetongluo groups (P>0.05). Huoxuetongluo decoction can promote the repairing and reconstruction of femoral head necrosis through increasing the level of insulin-like growth factor-1, but the level of insulin-like growth factor-1 wil not increased with the increasing of the dose of Huoxuetongluo decoction when the dose reached to a certain level.