CAJ | 학술논문

应用SRY原位杂交技术检测肝脏干细胞移植肝内种植情况☆
응용SRY원위잡교기술검측간장간세포이식간내충식정황☆
Y-chromosome in situ hybridization technology for detection of transplanted hepatic stem cells in the liver graft

万方数据

中国组织工程研究中國組織工程研究 중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2012年 53期 9881-9886 ,共6页

李铸%陈娟%李学华%刘志恒%刘桂杰%张学利%高延超%孙会东%纪丕友%张金良%李立

리주%진연%리학화%류지항%류계걸%장학리%고연초%손회동%기비우%장금량%리립

肝移植%大鼠%肝脏干细胞%细胞种植%SRY 原位杂交肝移植%大鼠%肝髒榦細胞%細胞種植%SRY 原位雜交
간이식%대서%간장간세포%세포충식%SRY 원위잡교

背景:以往干细胞种植后的定植示踪技术有磁探针标记、荧光示踪标记法、慢病毒感染荧光标记法及性别交叉 Y 染色体原位杂交检测法,各有优缺点.目的:应用 SRY 原位杂交技术观察受体来源肝脏干细胞(肝卵圆细胞)在移植肝内的定植情况.方法:二袖套法建立大鼠原位肝移植模型.供体为雌性 DA 大鼠,受体为雌性 Lewis 大鼠,设受体大鼠60只,肝卵圆细胞为 Lewis 大鼠来源的雄性细胞,移植中供肝种植肝卵圆细胞悬液.应用 SRY 原位杂交染色技术观察受体来源的雄性肝卵圆细胞在雌性移植肝内的定植情况.结果与结论:经门静脉和肝动脉注射种植的肝卵圆细胞及其分化细胞主要定植在移植肝内中央静脉及汇管区周围,应用 SRY 原位杂交染色技术可以有效观察到受体来源肝脏干细胞在移植肝内的定植情况.
배경:이왕간세포충식후적정식시종기술유자탐침표기、형광시종표기법、만병독감염형광표기법급성별교차 Y 염색체원위잡교검측법,각유우결점.목적:응용 SRY 원위잡교기술관찰수체래원간장간세포(간란원세포)재이식간내적정식정황.방법:이수투법건립대서원위간이식모형.공체위자성 DA 대서,수체위자성 Lewis 대서,설수체대서60지,간란원세포위 Lewis 대서래원적웅성세포,이식중공간충식간란원세포현액.응용 SRY 원위잡교염색기술관찰수체래원적웅성간란원세포재자성이식간내적정식정황.결과여결론:경문정맥화간동맥주사충식적간란원세포급기분화세포주요정식재이식간내중앙정맥급회관구주위,응용 SRY 원위잡교염색기술가이유효관찰도수체래원간장간세포재이식간내적정식정황.
BACKGROUND: The colonization tracer technology after stem cel s transplantation includes magnetic probe labeling, fluorescent tracer method, lentivirus infections fluorescent labeling and Y chromosome in situ hybridization technology, and each method has its own disadvantages and advantages. OBJECTIVE: To observe the transplantation conditions of hepatic stem cel s (hepatic oval cel s) in the liver graft by Y-chromosome in situ hybridization technology. METHODS: Rat orthotropic liver transplantation models were established by using Kamada two-cuff technique. Female DA rats served as donors, and 60 female Lewis rats were used as recipients. Hepatic oval cel s was originated from male Lewis rats and implanted to the liver graft during liver transplantation. Y-chromosome in situ hybridization technology was used to detect hepatic stem cel s transplantation conditions in the liver graft. RESULTS AND CONCLUSION: Hepatic oval cel s and differentiated cel s mainly distributed around central vein area and portal area in the liver graft. The transplantation conditions of recipient derived hepatic stem cel s in the liver graft were effectively detected by Y-chromosome in situ hybridization technology.