CAJ | 학술논문

背景:小肠移植慢性排斥反应的发生是影响其长远预后的重要因素.目的:观察环孢素对于小肠移植物中转化生长因子β1表达的影响.方法:近交系 F344(RT11vr)大鼠和 Lewis(RT11)大鼠作为小肠移植的供体和受体,利用显微外科技术构建异系大鼠小肠移植模型,分别在建模后7,28,60 d 进行移植物活检,常规病理学检测,应用 Real time-PCR 技术检测移植物内转化生长因子β1因子 mRNA 转录水平,并采用免疫组织化学方法对转化生长因子β1的表达进行定位.结果与结论:在建模后7-28 d 应用环孢素的过程中转化生长因子β1 mRNA 转录水平呈现明显减低趋势;在28-60 d,停用环孢素后该因子表达则明显升高.说明环孢素对于异系小肠移植中转化生长因子β1的表达有一定的抑制作用.
배경:소장이식만성배척반응적발생시영향기장원예후적중요인소.목적:관찰배포소대우소장이식물중전화생장인자β1표체적영향.방법:근교계 F344(RT11vr)대서화 Lewis(RT11)대서작위소장이식적공체화수체,이용현미외과기술구건이계대서소장이식모형,분별재건모후7,28,60 d 진행이식물활검,상규병이학검측,응용 Real time-PCR 기술검측이식물내전화생장인자β1인자 mRNA 전록수평,병채용면역조직화학방법대전화생장인자β1적표체진행정위.결과여결론:재건모후7-28 d 응용배포소적과정중전화생장인자β1 mRNA 전록수평정현명현감저추세;재28-60 d,정용배포소후해인자표체칙명현승고.설명배포소대우이계소장이식중전화생장인자β1적표체유일정적억제작용.
BACKGROUND: The chronic rejection of intestinal graft is the important factor affecting the long term prognosis. OBJECTIVE: To observe the effect of cyclosporine A on the expression of transforming growth factor-β1 in intestinal graft in rats. METHODS: Al ogeneic smal intestine transplantation was performed in rats by microsurgical technology with the donor of F344(RT11vr) rats and the recipient of Lewis(RT11) rats. The graft biopsy and routine pathologic examination were performed at 7, 28 and 60 days after modeling. mRNA transcription level of transforming growth factor-β1 was detected by real time-PCR. Immunohistochemical staining was also performed to detect the expression of transforming growth factor-β1. RESULTS AND CONCLUSION: The mRNA transcription level of transforming growth factor-β1 was significantly decreased during the application of cyclosporine A at 7-28 days after modeling. At 28-60 days after modeling, the mRNA transcription level was significantly increased after termination of cyclosporine A. Cyclosporine A can inhibit the expression of transforming growth factor-β1 in al ogeneic smal intestine transplantation.