医药前沿
醫藥前沿
의약전연
YIAYAO QIANYAN
2012年
32期
93-95
,共3页
参芪二甲丸%高效液相色谱%蒸发光散射检测法%黄芪甲苷
參芪二甲汍%高效液相色譜%蒸髮光散射檢測法%黃芪甲苷
삼기이갑환%고효액상색보%증발광산사검측법%황기갑감
Shenqierjia Capsules%HPLC%ELSD%Astragaloside Ⅳ
目的建立参芪二甲丸中黄芪甲苷的 HPLC-ELSD 测定方法.方法采用高效液相色谱-蒸发光散射检测法(HPLC-ELSD),色谱柱为 Agilent-C18(4.6 mm×150 mm,5μm),流动相乙腈-水(30∶70),柱温35℃,流速1.6L?min-1.结果测定了四批参芪二甲丸中黄芪甲苷的含量,建立了该制剂中黄芪甲苷的含量测定方法.结论该方法简便、快速、灵敏、准确、重复性好,专属性强,可作为参芪二甲丸的质量控制方法.
目的建立參芪二甲汍中黃芪甲苷的 HPLC-ELSD 測定方法.方法採用高效液相色譜-蒸髮光散射檢測法(HPLC-ELSD),色譜柱為 Agilent-C18(4.6 mm×150 mm,5μm),流動相乙腈-水(30∶70),柱溫35℃,流速1.6L?min-1.結果測定瞭四批參芪二甲汍中黃芪甲苷的含量,建立瞭該製劑中黃芪甲苷的含量測定方法.結論該方法簡便、快速、靈敏、準確、重複性好,專屬性彊,可作為參芪二甲汍的質量控製方法.
목적건립삼기이갑환중황기갑감적 HPLC-ELSD 측정방법.방법채용고효액상색보-증발광산사검측법(HPLC-ELSD),색보주위 Agilent-C18(4.6 mm×150 mm,5μm),류동상을정-수(30∶70),주온35℃,류속1.6L?min-1.결과측정료사비삼기이갑환중황기갑감적함량,건립료해제제중황기갑감적함량측정방법.결론해방법간편、쾌속、령민、준학、중복성호,전속성강,가작위삼기이갑환적질량공제방법.
OBJECTIVE To build up a method for determination of astragaloside Ⅳ in shenqierjia capsules by HPLC-ELSD. METHODS HPLC-ELSD was used in the quantitative analysis with Agilent-C18 chromatography column (4.6 mm×150mm, 5μm) and acetonitrile-water (30:70) as a mobile phase. The column temperature was set to 35℃ .The flow rate of mobile phase was 1.6L?min-1.RESULTS Determining astragaloside Ⅳ in four groups of shenqierjia capsules, then develop a method for determination of astragaloside Ⅳ in sample. CONCLUSION This method is simple、rapid、reproducible and can be regarded as the quality control method of shenqierjia capsules.
