CAJ | 학술논문

目的建立一种测定人唾液中对乙酰氨基酚浓度的HPLC法.方法受试者服用对乙酰氨基酚片,分别于不同时间点采集受试者的唾液,唾液样品用1.5倍量的10﹪高氯酸和0.5倍量的乙腈沉淀蛋白.色谱柱为Dikma Technologies DiamonsilTM C18(5μm,250×4.6mm),流动相为甲醇-水(20:80,V/V),流速为1ml?min-1,柱温为室温,紫外检测波长245 nm.结果唾液中内源性物质对样品测定无干扰.本方法线性范围为0.025-12.5μg?mL-1(r=0.9999),最低定量浓度为0.025μg?mL-1,方法回收率为99.02﹪-102.19﹪,精密度等均符合方法学要求.结论本法简便、准确,适用于对乙酰氨基酚唾药浓度测定.
목적건립일충측정인타액중대을선안기분농도적HPLC법.방법수시자복용대을선안기분편,분별우불동시간점채집수시자적타액,타액양품용1.5배량적10﹪고록산화0.5배량적을정침정단백.색보주위Dikma Technologies DiamonsilTM C18(5μm,250×4.6mm),류동상위갑순-수(20:80,V/V),류속위1ml?min-1,주온위실온,자외검측파장245 nm.결과타액중내원성물질대양품측정무간우.본방법선성범위위0.025-12.5μg?mL-1(r=0.9999),최저정량농도위0.025μg?mL-1,방법회수솔위99.02﹪-102.19﹪,정밀도등균부합방법학요구.결론본법간편、준학,괄용우대을선안기분타약농도측정.
Objective A HPLC method was established for the determination of acetaminophen in human saliva. Method Take saliva samples of healthy subjects after oral administration of acetaminophen tablets in different time. Proteins in saliva samples were precipitated by 1.5 times of 10﹪ perchloric acid and 0.5 times of acetonitrile . A Dikma Technologies DiamonsilTM C18 column (5μm,250×4.6mm) was used with the mobile phase of methanol-acetonitrile (20 :80,V/V) at a flow rate of 1.0 mL ? min-1. The detection wavelength was at 245nm. Results The endogenous substance in saliva did not interfere with the determination of the samples. The linear range was 0.025 -12.5μg ? mL-1 (r=0. 9999), and the limit of quantitation was 0.025μg ? mL-1. The method recovery was 99.02﹪- 102.19﹪.The degree of precision also appeared good. Conclusion The method is simple and accurate. It is suitable for the determination of acetaminophen in human saliva.