CAJ | 학술논문

目的探讨银杏黄酮与阿托伐他汀联用对高血脂小鼠的降脂作用.方法选取雄性金黄地鼠随机分配成空白对照组、高脂模型组、银杏黄酮组、阿托伐他汀组、银杏黄酮与阿托伐他汀复方给药组(简称复方给药组).空白对照组喂养普通饲料,其余各实验组喂养高脂饲料.实验过程中记录小鼠体重变化,给药六周后断头处死分离血清,进行血脂成分总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL)、低密度脂蛋白胆固醇(LDL)的检测.取其肝脏、肾脏及腹主动脉组织,观察肝脏表面的病理改变,并称量肝、肾的重量计算肝、肾指数.将各组织制成石蜡切片,肝、肾组织进行HE染色后在显微镜下观察组织形态变化;腹主动脉组织进行油红O染色后显微镜下观察组织形态变化,并应用Motic Digilab Ⅱ-Client图像分析软件测量腹主动脉内膜脂质的厚度,比较不同给药组的腹主动脉内膜的变化.结果①高血脂模型组与空白组比较体重呈现显著的快速增长,复方给药组与模型组比较可以极显著的抑制金黄地鼠体重的增加(P <0.01).②高脂模型组小鼠血清TC、TG、LDL含量与空白组比较有显著性差异(P<0.05).阿托伐他汀组、复方给药组分别与模型组比较血脂各成分均有显著性变化(P<0.01或0.001)③各给药组肝脏指数与模型组比较无显著差异,与空白组比较有显著的差异(P<0.05).各组动物肾脏指数与空白组比较均无显著差异(P>0.05).④银杏黄酮单用药组与模型组比较脂质厚度变化不显著(P>0.05);阿托伐他汀组和复方给药组分别与模型组比较脂质厚度具有显著性变化(P<0.05).结论银杏黄酮与阿托伐他汀复方药物具有明显提高降脂效力的作用.
목적탐토은행황동여아탁벌타정련용대고혈지소서적강지작용.방법선취웅성금황지서수궤분배성공백대조조、고지모형조、은행황동조、아탁벌타정조、은행황동여아탁벌타정복방급약조(간칭복방급약조).공백대조조위양보통사료,기여각실험조위양고지사료.실험과정중기록소서체중변화,급약륙주후단두처사분리혈청,진행혈지성분총담고순(TC)、감유삼지(TG)、고밀도지단백담고순(HDL)、저밀도지단백담고순(LDL)적검측.취기간장、신장급복주동맥조직,관찰간장표면적병리개변,병칭량간、신적중량계산간、신지수.장각조직제성석사절편,간、신조직진행HE염색후재현미경하관찰조직형태변화;복주동맥조직진행유홍O염색후현미경하관찰조직형태변화,병응용Motic Digilab Ⅱ-Client도상분석연건측량복주동맥내막지질적후도,비교불동급약조적복주동맥내막적변화.결과①고혈지모형조여공백조비교체중정현현저적쾌속증장,복방급약조여모형조비교가이겁현저적억제금황지서체중적증가(P <0.01).②고지모형조소서혈청TC、TG、LDL함량여공백조비교유현저성차이(P<0.05).아탁벌타정조、복방급약조분별여모형조비교혈지각성분균유현저성변화(P<0.01혹0.001)③각급약조간장지수여모형조비교무현저차이,여공백조비교유현저적차이(P<0.05).각조동물신장지수여공백조비교균무현저차이(P>0.05).④은행황동단용약조여모형조비교지질후도변화불현저(P>0.05);아탁벌타정조화복방급약조분별여모형조비교지질후도구유현저성변화(P<0.05).결론은행황동여아탁벌타정복방약물구유명현제고강지효력적작용.
Objective:TO investigate the lipid-lowering effect of Ginkgo flavone combined with atorvastatin on hyperlipemia mice. Methods: selected male golden hamsters were randomly assigned into blank control group, Hyperlipidemia model group, Ginkgo flavone group, atorvastatin group, Ginkgo biloba flavone compound with atorvastatin group (referred to as the compound administration group). Blank control group feeding ordinary feed, the remainder of the experimental group fed with high fat diet. Recorded weight In the process of experiment, meanwhile decapitated Golden hamster after six weeks and separated the serum total cholesterol, detect the content of serum lipid components (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL), low density lipoprotein cholesterol (LDL) detection. Dissect the liver, kidney and aorta tissue ,then observe the pathological changes of liver surface and weighing liver and kidney ,then calculate liver and kidney index. The tissues were made into paraffin section ,obsreved the morphological changes of tissues under microscope after staining with HE; Abdominal aorta stained with oil red O were observed changes of morphology under microscope , and use Motic Digilab Ⅱ -Client image analysis software for measuring abdominal aortic intimal lipid thickness,then compared abdominal aortic intimal changes.of the different administration group . Results: ① The weight of hyperlipidemia model group mice obviously rapid growth compared with normal group, compound drug treatment group can significantly inhibit the hamster weight gain compared with the model group(P<0.01). ② Serum TC, TG, LDL content of hyperlipidemia model mice had significant difference comparied with blank group (P<0.05).Atorvastatin group, compound drug treatment group had significant changes compared with model lipid components(P<0.01 or 0.001);③ The liver index of each group had no significant difference compared with the model group ,while there were significant differences compared with control group(P<0.05).The kidney index of each group animal showed no significant difference compared with control group (P>0.05);④ The lipid thickness of single ginkgo flavone group is not significant change compared with model (P>0.05); lipid thickness of atorvastatin group and the compound group has significant changes compared with model group(P<0.05). Conclusion:Ginkgo biloba flavone combinated with atorvastatin drugs can apparently improve the effect of lipid-lowering.