医药前沿
醫藥前沿
의약전연
YIAYAO QIANYAN
2013年
3期
124-126
,共3页
罗洪顺%李华娣%黄志骢%李莉
囉洪順%李華娣%黃誌驄%李莉
라홍순%리화제%황지총%리리
亚洲鼻炎丸%防风%甘草%升麻素苷%TLC%HPLC%质量控制
亞洲鼻炎汍%防風%甘草%升痳素苷%TLC%HPLC%質量控製
아주비염환%방풍%감초%승마소감%TLC%HPLC%질량공제
Yazhou Biyan Wan%Saposhnikoviae Radlx%Glycyrrhizae Radix Et Rhixoma%Prim-O-glucosylcimifugin%TLC%HPLC quality control
建立亚洲鼻炎丸(防风、甘草等)的质量控制方法.方法采用TLC法对防风和甘草进行定性鉴别;采用HPLC去对升麻素苷进行定量分析.色谱柱为Agela technologies C18(4.6×150mm 5μm),流动相:以甲醇-水(27:73);体积流量为1.0ml/min,检测波长为254nm.结果薄层色谱斑点清晰,阴性对照无干扰;升麻素苷进样量在0.0424~0.424μg范围内线性关系良好(r=1.0000),平均加样回收率为102.3%,RSD为1.15%.结论所建立的方法能准确可靠地进行定性、定量检测,重复性好,可作为本品的质量控制方法.
建立亞洲鼻炎汍(防風、甘草等)的質量控製方法.方法採用TLC法對防風和甘草進行定性鑒彆;採用HPLC去對升痳素苷進行定量分析.色譜柱為Agela technologies C18(4.6×150mm 5μm),流動相:以甲醇-水(27:73);體積流量為1.0ml/min,檢測波長為254nm.結果薄層色譜斑點清晰,陰性對照無榦擾;升痳素苷進樣量在0.0424~0.424μg範圍內線性關繫良好(r=1.0000),平均加樣迴收率為102.3%,RSD為1.15%.結論所建立的方法能準確可靠地進行定性、定量檢測,重複性好,可作為本品的質量控製方法.
건립아주비염환(방풍、감초등)적질량공제방법.방법채용TLC법대방풍화감초진행정성감별;채용HPLC거대승마소감진행정량분석.색보주위Agela technologies C18(4.6×150mm 5μm),류동상:이갑순-수(27:73);체적류량위1.0ml/min,검측파장위254nm.결과박층색보반점청석,음성대조무간우;승마소감진양량재0.0424~0.424μg범위내선성관계량호(r=1.0000),평균가양회수솔위102.3%,RSD위1.15%.결론소건립적방법능준학가고지진행정성、정량검측,중복성호,가작위본품적질량공제방법.
AIM To establish the quality control methods of Yazhou Biyan Wan(Saposhnikoviae Radlx,Glycyrrhizae Radix Et Rhixoma etc.).METHODS TLC was prformed t identify Saposhnikoviae Radlx,Glycyrrhizae Radix Et Rhixoma in Gukang capsules.HPLC was used to dtrmine the contents of Prim-O-glucosylcimifugin .The gradint elutiou was performed on a Agela technologies C18 column(4.6×150mm 5μm);with methanol-water(27:73).The flow was 1.0mL/min.the detection wavelength was set at 254nm. RESULTS The spots on TLC plates were clear without interference. The calibration curve was linear within the range of 0.0424~0.424μg for Prim-O-glucosylcimifugin ,and the average recovery was 102.3﹪(RSD was 1.15﹪). CONCLUSION The methods are simple ,reliable,accurate,reproducible,and suitable for the quality control of Gukang capsules.
