CAJ | 학술논문

温度是影响香根草在温、寒带推广应用的主要限制因子.为提高香根草的耐寒性,利用分子遗传学手段将拟南芥基因组中的转录激活因子基因AtCBF3导入香根草中,实现种质改良.本研究从野生型拟南芥哥伦比亚Columbia植株中克隆出AtCBF3,经酶切连接构建了表达载体pCAMBIA1301/ AtCBF3.再通过农杆菌介导法导入外源基因后,在含抗生素培养基上筛选获得抗性植株,后经PCR和越冬试验后证实获得了抗寒性增强的转基因香根草植株.
온도시영향향근초재온、한대추엄응용적주요한제인자.위제고향근초적내한성,이용분자유전학수단장의남개기인조중적전록격활인자기인AtCBF3도입향근초중,실현충질개량.본연구종야생형의남개가륜비아Columbia식주중극륭출AtCBF3,경매절련접구건료표체재체pCAMBIA1301/ AtCBF3.재통과농간균개도법도입외원기인후,재함항생소배양기상사선획득항성식주,후경PCR화월동시험후증실획득료항한성증강적전기인향근초식주.
Temperature is the main restricted factor for distribution and application of vetiver (Vetiveria zizanioides) in Temperate and Frigid Zone. To improve its chilling resistance, we established an Agrobacterium-mediated gen transfer system, in which a gene encoding transcriptional activator CBF3 extracted from Arabidopsis was transferred into the vetiver calli. The research results showed that the transgenic vetiver seedlings which overexpressed CBF3 were more tolerant to low temperature stress.