玉林师范学院学报
玉林師範學院學報
옥림사범학원학보
Journal of Yulin Teachers College
2012年
5期
61-64
,共4页
乳糖%IPTG%诱导%降血糖
乳糖%IPTG%誘導%降血糖
유당%IPTG%유도%강혈당
lactose%IPTG%induction%hypoglycemic
对重组E.coli BL21(DE3)表达降血糖药物胰升血糖素样肽-1类似物的乳糖诱导方式进行了研究.结果表明诱导剂乳糖分批添加在生物量与蛋白表达量上均优于一次性添加方式,因为乳糖可被细菌作为碳源利用,分批添加乳糖诱导的目的蛋白表达量高达48.0%.研究结果证明乳糖分批流加可用于高密度发酵表达重组GLP-1类似物,并对其它重组基因工程药物的生产具有指导意义.
對重組E.coli BL21(DE3)錶達降血糖藥物胰升血糖素樣肽-1類似物的乳糖誘導方式進行瞭研究.結果錶明誘導劑乳糖分批添加在生物量與蛋白錶達量上均優于一次性添加方式,因為乳糖可被細菌作為碳源利用,分批添加乳糖誘導的目的蛋白錶達量高達48.0%.研究結果證明乳糖分批流加可用于高密度髮酵錶達重組GLP-1類似物,併對其它重組基因工程藥物的生產具有指導意義.
대중조E.coli BL21(DE3)표체강혈당약물이승혈당소양태-1유사물적유당유도방식진행료연구.결과표명유도제유당분비첨가재생물량여단백표체량상균우우일차성첨가방식,인위유당가피세균작위탄원이용,분비첨가유당유도적목적단백표체량고체48.0%.연구결과증명유당분비류가가용우고밀도발효표체중조GLP-1유사물,병대기타중조기인공정약물적생산구유지도의의.
The effects of different lactose-induction ways on cell growth and expression of hypoglycemic drug GLP-1 derivate in recombinant E.coli BL21(DE3) were investigated. The results demonstrated it was better to add the lactose in bathes than to add in one time in cell density and the expression level of target protein, for lactose can be used as carbon source by the bacteria. The expression level of target protein reached up to 48.0%(the percentage of target protein of total cellular protein). The results showed that the batch addition of lactose could be used in high density fermentation expression of recombinant GLP-1 derivate, and gave reference to other recombinant gene-engineering drugs' production.
