CAJ | 학술논문

背景与目的:超抗原葡萄球菌肠毒素A(staphylococcal enterotoxin A,SEA)在体内、外以及临床的膀胱灌注治疗试验中对膀胱癌均具有明显的抑瘤效应,本研究旨在探讨单抗靶向的超抗原SEA体内对人膀胱癌的抑瘤作用.方法:以腹腔注射人外周血单个核细胞、皮下注射人膀胱癌BIU87细胞建立人免疫重建荷人膀胱癌SCID小鼠复合模型,再将模型小鼠随机分为对照组、SEA组及单抗靶向SEA实验组,分别予以PBS、SEA及单抗靶向SEA瘤周注射,以SonoSite彩超在建模后6周内检测小鼠皮下移植瘤体积、瘤体血流.结果:单抗靶向SEA组瘤体体积、瘤体血流均小于对照组及SEA组,在建模后4、5、6周差异均有统计学意义(P<0.05).结论:单抗靶向的超抗原SEA对膀胱癌皮下移植瘤生长的抑制作用更强,可能与其进一步减少瘤体血流有关.
배경여목적:초항원포도구균장독소A(staphylococcal enterotoxin A,SEA)재체내、외이급림상적방광관주치료시험중대방광암균구유명현적억류효응,본연구지재탐토단항파향적초항원SEA체내대인방광암적억류작용.방법:이복강주사인외주혈단개핵세포、피하주사인방광암BIU87세포건립인면역중건하인방광암SCID소서복합모형,재장모형소서수궤분위대조조、SEA조급단항파향SEA실험조,분별여이PBS、SEA급단항파향SEA류주주사,이SonoSite채초재건모후6주내검측소서피하이식류체적、류체혈류.결과:단항파향SEA조류체체적、류체혈류균소우대조조급SEA조,재건모후4、5、6주차이균유통계학의의(P<0.05).결론:단항파향적초항원SEA대방광암피하이식류생장적억제작용경강,가능여기진일보감소류체혈류유관.
Background and purpose:Staphylococcal enterotoxin A (SEA) is potent T-cell stimulators which will produce significant tumor inhibition on bladder cancer. This study was to investigate the anti-tumor effect of monoclonal antibody targeted superantigen SEA on human bladder cancer in vivo. Methods:Hu-PBL-SCID mice of human bladder carcinoma, which were transplanted with human peripheral blood lymphocyte intraperitoneally and injected with human bladder carcinoma cells BIU87 subcutaneously, were divided randomly into 3 groups:control, SEA and monoclonal antibody targeted SEA group, subject to subcutaneous injection with PBS, SEA, or monoclonal antibody targeted SEA surrounding the xenograft respectively. Tumor size and blood flow were monitored under the SonoSite ultrasound system. Results:Compared with control and SEA groups, the tumor size and blood flow of monoclonal antibody targeted SEA group were significantly depressed (P<0.05). Conclusion:Monoclonal antibody targeted SEA significantly depresses the xenograft growth, possibly through the down-regulation of tumor blood flow.