CAJ | 학술논문

目的观察杏鲍菇多糖对D-半乳糖衰老模型小鼠的影响.方法 ICR小鼠72只,随机分为6组:多糖低剂量组、多糖中剂量组、多糖高剂量组、脑复康组、模型组、正常对照组,每组12只.建立D-半乳糖致小鼠衰老模型.6周后行Y型迷宫测试,测定脏器指数,采用黄嘌呤氧化酶法、TBA比色法测定大脑超氧化物歧化酶(SOD)活性和丙二醛含量,Sohal法测定小鼠大脑组织中脂褐质含量,双波长薄层扫描仪测定全脑谷氨酸的含量.结果与模型组相比,多糖中、高剂量组Y型迷宫成绩提高(P<0.05),脑SOD活性上升,丙二醛、脂褐质含量降低;多糖高剂量组脑指数和肾指数上升,脑组织谷氨酸含量降低(P<0.05).结论
목적관찰행포고다당대D-반유당쇠로모형소서적영향.방법 ICR소서72지,수궤분위6조:다당저제량조、다당중제량조、다당고제량조、뇌복강조、모형조、정상대조조,매조12지.건립D-반유당치소서쇠로모형.6주후행Y형미궁측시,측정장기지수,채용황표령양화매법、TBA비색법측정대뇌초양화물기화매(SOD)활성화병이철함량,Sohal법측정소서대뇌조직중지갈질함량,쌍파장박층소묘의측정전뇌곡안산적함량.결과여모형조상비,다당중、고제량조Y형미궁성적제고(P<0.05),뇌SOD활성상승,병이철、지갈질함량강저;다당고제량조뇌지수화신지수상승,뇌조직곡안산함량강저(P<0.05).결론
Objective To study the effect of Pleurotus eryngii polysaccharide on aging mice induced by D-galactose (D-gal). Methods 72 ICR mice were divided into high, moderate, and low dosages (of polysaccharide) groups, piracetam group, model group, and control group, 12 in each group. The model of aging mice was induced with D-gal. They were tested with Y-maze, and the contents of superoxide dismutase (SOD), malondialdehyde (MDA), lipofuscin and L-glutamate in cerebrum were determined 6 weeks after model. Results Com-pared with the model group, the achievement of Y-maze improved, the activities of SOD increased and the content of MDA and lipofuscin decreasd in high and moderate dosage groups (P<0.05), while the mass of brain and kidney increased and the L-glutamate decreased in the high dosage group (P<0.05). Conclusion Pleurotus eryngii polysaccharide can ameliorate aging of mice induced by D-gal.