中国康复理论与实践
中國康複理論與實踐
중국강복이론여실천
CHINESE JOURNAL OF REHABILITATION THEORY & PRACTICE
2012年
12期
1112-1115
,共4页
何斌%吴鸿浩%吕金如%孙昊%吴昊%蒋雷%王淦楠%胡德亮%张劲松%陈彦
何斌%吳鴻浩%呂金如%孫昊%吳昊%蔣雷%王淦楠%鬍德亮%張勁鬆%陳彥
하빈%오홍호%려금여%손호%오호%장뢰%왕감남%호덕량%장경송%진언
脑缺血%人参皂甙Rg1%谷胱甘肽%细胞凋亡%海马
腦缺血%人參皂甙Rg1%穀胱甘肽%細胞凋亡%海馬
뇌결혈%인삼조대Rg1%곡광감태%세포조망%해마
cerebral ischemia%Ginsenoside Rg1%glutathion%apoptosis%hippocampus
目的观察人参皂甙Rg1对大鼠海马神经元缺糖氧/复糖氧后谷胱甘肽(GSH)和谷胱甘肽过氧化物酶(GPx)的影响.方法海马神经元培养8~10 d,随机分为正常对照组、模型组、人参皂甙Rg1低、中、高剂量组(5μmol/L,20μmol/L,60μmol/L).建立大鼠海马神经元缺糖氧/复糖氧模型,复糖氧后6 h以生物化学法观察各组海马神经元GSH含量和GPx活性的变化;复糖氧后24 h以Hochest染色法检测细胞凋亡,并检测各组海马神经元四甲基偶氮唑盐(MTT)代谢率.结果与模型组相比,人参皂甙Rg1中、高剂量组海马神经元GSH含量、GPx活性显著升高,凋亡显著减少,MTT代谢率显著提高(P<0.001),人参皂甙Rg1低剂量组变化不明显(P>0.05).结论人参皂甙Rg1可通过提高缺糖氧神经元GSH含量和GPx活性,发挥脑保护作用.
目的觀察人參皂甙Rg1對大鼠海馬神經元缺糖氧/複糖氧後穀胱甘肽(GSH)和穀胱甘肽過氧化物酶(GPx)的影響.方法海馬神經元培養8~10 d,隨機分為正常對照組、模型組、人參皂甙Rg1低、中、高劑量組(5μmol/L,20μmol/L,60μmol/L).建立大鼠海馬神經元缺糖氧/複糖氧模型,複糖氧後6 h以生物化學法觀察各組海馬神經元GSH含量和GPx活性的變化;複糖氧後24 h以Hochest染色法檢測細胞凋亡,併檢測各組海馬神經元四甲基偶氮唑鹽(MTT)代謝率.結果與模型組相比,人參皂甙Rg1中、高劑量組海馬神經元GSH含量、GPx活性顯著升高,凋亡顯著減少,MTT代謝率顯著提高(P<0.001),人參皂甙Rg1低劑量組變化不明顯(P>0.05).結論人參皂甙Rg1可通過提高缺糖氧神經元GSH含量和GPx活性,髮揮腦保護作用.
목적관찰인삼조대Rg1대대서해마신경원결당양/복당양후곡광감태(GSH)화곡광감태과양화물매(GPx)적영향.방법해마신경원배양8~10 d,수궤분위정상대조조、모형조、인삼조대Rg1저、중、고제량조(5μmol/L,20μmol/L,60μmol/L).건립대서해마신경원결당양/복당양모형,복당양후6 h이생물화학법관찰각조해마신경원GSH함량화GPx활성적변화;복당양후24 h이Hochest염색법검측세포조망,병검측각조해마신경원사갑기우담서염(MTT)대사솔.결과여모형조상비,인삼조대Rg1중、고제량조해마신경원GSH함량、GPx활성현저승고,조망현저감소,MTT대사솔현저제고(P<0.001),인삼조대Rg1저제량조변화불명현(P>0.05).결론인삼조대Rg1가통과제고결당양신경원GSH함량화GPx활성,발휘뇌보호작용.
Objective To explore the effect of Ginsenoside Rgl on glutathion (GSH) level and glutathion peroxidase (GPx) activity after oxygen-glucose deprivation/reperfusion in cultured hippocampal cells. Methods The model of oxygen-glucose deprivation and reperfusion were established with the hippocampal neurons of rats. They were randomly divided into control group, model group and Ginsenoside Rgl treatment groups (5 μmol/L, 20 μmol/L, 60 μmol/L). The GSH level and GPx activity were measured 6 h after reperfusion. The apoptosis and the metabolic rate of methyl thiazolyl tetrazolium (MTT) were detected 24 h after reperfusion. Results Compared with model group, the GSH level, GPx activity, and metabolic rate of MTT improved (P<0.001), and the apoptosis decreased in the Ginsenoside Rgl groups (P<0.001) except with the dosage of 5 μmol/L (P>0.05). Conclusion Ginsenoside Rgl can protect the brain from ischemia by increasing the GSH level and GPx activity.
