CAJ | 학술논문

利用磷盐触发2,4,5-三苯基吡喃盐开环,合成了一种可用于线粒体成像荧光探针的新型的共轭膦盐TPP.该化合物10μmol/L在纯乙腈中几乎不发光,但增加溶度或者增加溶液水含量后,共轭膦盐TPP的荧光强度显著增加,表明该化合物在水溶液中具有优良的聚集诱导荧光增强的性质.通过场发射扫描电镜观察,共轭膦盐TPP能够自组装形成荧光纳米粒子.利用共聚焦显微镜的双通道检测成像实验证实了该化合物具有高度的线粒体选择性.
이용린염촉발2,4,5-삼분기필남염개배,합성료일충가용우선립체성상형광탐침적신형적공액련염TPP.해화합물10μmol/L재순을정중궤호불발광,단증가용도혹자증가용액수함량후,공액련염TPP적형광강도현저증가,표명해화합물재수용액중구유우량적취집유도형광증강적성질.통과장발사소묘전경관찰,공액련염TPP능구자조장형성형광납미입자.이용공취초현미경적쌍통도검측성상실험증실료해화합물구유고도적선립체선택성.
A new conjugated phosphonium salt TPP, which can be used as fluorescence probes to image mitochondria in cells, has been synthesized readily from phosphine-triggered ring-opening of 2,4,5-triphenylpyrylium salt. When TPP was dissolved in CH3CN to dilute solution (10 μmol/L), almost no fluorescence was observed. Increasing the concentration of the solution or the fractions of water in mixture dramatically enhanced the fluorescent intensity, which indicates the aggregation-induced emission enhancement (AIEE) feature. The shape of nanoparticles was observed by field emission scanning electron microscopy (FE-SEM). Confocal fluorescence images of live HEK-293 cells demonstrate that TPP could yield high intracellular selectivity toward mitochondria.