CAJ | 학술논문

目的探讨冷冻联合全反式维甲酸(ATRA)抑制大鼠C6脑胶质瘤细胞的增殖及其机制.方法在120只Wistar大鼠建立C6脑胶质瘤模型,并随机将其平均分为冷冻治疗、ATRA治疗、冷冻联合ATRA治疗及对照组.接种C6细胞第22天MRI检测10只动物的胶质瘤体积的变化,每组并处死10只动物取肿瘤组织,采用免疫组化法检测p27kip1蛋白的表达;观察余下10只荷瘤鼠的生存期.结果各治疗组较对照组肿瘤体积明显缩小(P<0.05),大鼠生存期明显延长,p27kip1蛋白的表达水平明显增多,以联合治疗组最为显著(P<0.05);p27kip1蛋白的表达与肿瘤体积呈明显负相关(P<0.05),与大鼠生存期呈明显正相关(P<0.05).结论与单一治疗相比,冷冻联合ATRA治疗能显著抑制大鼠C6胶质瘤细胞增殖,其机制可能与其表达p27kip1蛋白的上调有关.
목적탐토냉동연합전반식유갑산(ATRA)억제대서C6뇌효질류세포적증식급기궤제.방법재120지Wistar대서건립C6뇌효질류모형,병수궤장기평균분위냉동치료、ATRA치료、냉동연합ATRA치료급대조조.접충C6세포제22천MRI검측10지동물적효질류체적적변화,매조병처사10지동물취종류조직,채용면역조화법검측p27kip1단백적표체;관찰여하10지하류서적생존기.결과각치료조교대조조종류체적명현축소(P<0.05),대서생존기명현연장,p27kip1단백적표체수평명현증다,이연합치료조최위현저(P<0.05);p27kip1단백적표체여종류체적정명현부상관(P<0.05),여대서생존기정명현정상관(P<0.05).결론여단일치료상비,냉동연합ATRA치료능현저억제대서C6효질류세포증식,기궤제가능여기표체p27kip1단백적상조유관.
Objective To study the inhibitory effect of cryotherapy combined with all -trans retinoic acid (ATRA) on the C6 glioma cell proliferation in the rats and its possible mechanism. Methods The models of cerebral glioma were established by intracerebral injection of C6 cells in 120 Wistar rats. One hundred and twenty the rats with C6 gliomas were randomly divided into four groups of 30 animals each, i.e. cryotherapy, ATRA treatment, cryotherapy combined with ATRA treatment and control groups. The tumours volumes were determined by MRI 22 days after the implanting of C6 cells. The expression of p27kip1 protein in the gliomas was detected by immunohistochemical staining. The survival time of the rats was observed. Results The tumor volume was significantly smaller, the levels of p27kip1 protein expressions were significantly higher, and the rats life span was significantly longer in the cryotherapy combined with ATRA treatment group than those in both the cryotherapy and ATRA treatment groups (P<0.05). The tumors volumes were significantly smaller, the levels of p27kip1 expression protein were significantly higher and the rats life span were significantly longer in all the treatment groups than those in the control group (P<0.05). The level of p27kip1 expression protein was negatively correlated with the tumorous size (P<0.05) and positively correlated with the rat life span (P<0.05). Conclusions The cryotherapy combined with ATRA can significantly inhibit the proliferation of C6 glioma cells compared to single cryotherapy or ATRA treatment through the upregulation of p27kip1 protein expression in rats.