中国男科学杂志
中國男科學雜誌
중국남과학잡지
CHINESE JOURNAL OF ANDROLOGY
2012年
11期
15-19
,共5页
张卫星**%李周%王瑞%贾东辉%迟凯凯
張衛星**%李週%王瑞%賈東輝%遲凱凱
장위성**%리주%왕서%가동휘%지개개
钴%塞尔托利细胞%抑制素β亚单位
鈷%塞爾託利細胞%抑製素β亞單位
고%새이탁리세포%억제소β아단위
cobalt%sertoli cells%inhibin-beta subunits
目的探讨不同剂量钴60(60Co)-γ射线致小鼠睾丸支持细胞结构及功能急性损伤的程度.方法40只健康雄性 KM 小鼠,采用随机数字表法平均分为4组(n=10):正常对照组(A 组)和60Co-γ射线双侧睾丸局部照射的3个不同剂量组,分别为剂量2Gy(B 组);剂量6Gy(C 组);剂量10Gy(D 组),下腹部均匀照射517s.照射完成48h 后,采用酶联免疫吸附法测量血清卵泡刺激素(follicle-stimulating hormone,FSH)、促黄体生成素(luteinizing hormone ,LH)及抑制素β(Inhibin β)水平;应用光学显微镜观察小鼠睾丸组织形态学变化;应用实时定量 PCR 法测定睾丸组织中 Inhibinβ mRNA相对表达量.结果60Co-γ射线照射组(B、C、D 组)较正常组(A 组)血清 FSH 水平显著提高(P <0.05),Inhibinβ水平显著降低(P <0.05);光镜下见睾丸支持细胞和生精细胞发生不同程度的损伤,以 D 组损伤程度最为明显;睾丸组织 Inhibinβ mRNA 表达水平显著降低,差异均有统计学意义(P <0.05).结论60Co-γ射线对睾丸支持细胞结构及功能有明显损伤作用,且损伤程度与照射剂量呈正相关性.
目的探討不同劑量鈷60(60Co)-γ射線緻小鼠睪汍支持細胞結構及功能急性損傷的程度.方法40隻健康雄性 KM 小鼠,採用隨機數字錶法平均分為4組(n=10):正常對照組(A 組)和60Co-γ射線雙側睪汍跼部照射的3箇不同劑量組,分彆為劑量2Gy(B 組);劑量6Gy(C 組);劑量10Gy(D 組),下腹部均勻照射517s.照射完成48h 後,採用酶聯免疫吸附法測量血清卵泡刺激素(follicle-stimulating hormone,FSH)、促黃體生成素(luteinizing hormone ,LH)及抑製素β(Inhibin β)水平;應用光學顯微鏡觀察小鼠睪汍組織形態學變化;應用實時定量 PCR 法測定睪汍組織中 Inhibinβ mRNA相對錶達量.結果60Co-γ射線照射組(B、C、D 組)較正常組(A 組)血清 FSH 水平顯著提高(P <0.05),Inhibinβ水平顯著降低(P <0.05);光鏡下見睪汍支持細胞和生精細胞髮生不同程度的損傷,以 D 組損傷程度最為明顯;睪汍組織 Inhibinβ mRNA 錶達水平顯著降低,差異均有統計學意義(P <0.05).結論60Co-γ射線對睪汍支持細胞結構及功能有明顯損傷作用,且損傷程度與照射劑量呈正相關性.
목적탐토불동제량고60(60Co)-γ사선치소서고환지지세포결구급공능급성손상적정도.방법40지건강웅성 KM 소서,채용수궤수자표법평균분위4조(n=10):정상대조조(A 조)화60Co-γ사선쌍측고환국부조사적3개불동제량조,분별위제량2Gy(B 조);제량6Gy(C 조);제량10Gy(D 조),하복부균균조사517s.조사완성48h 후,채용매련면역흡부법측량혈청란포자격소(follicle-stimulating hormone,FSH)、촉황체생성소(luteinizing hormone ,LH)급억제소β(Inhibin β)수평;응용광학현미경관찰소서고환조직형태학변화;응용실시정량 PCR 법측정고환조직중 Inhibinβ mRNA상대표체량.결과60Co-γ사선조사조(B、C、D 조)교정상조(A 조)혈청 FSH 수평현저제고(P <0.05),Inhibinβ수평현저강저(P <0.05);광경하견고환지지세포화생정세포발생불동정도적손상,이 D 조손상정도최위명현;고환조직 Inhibinβ mRNA 표체수평현저강저,차이균유통계학의의(P <0.05).결론60Co-γ사선대고환지지세포결구급공능유명현손상작용,차손상정도여조사제량정정상관성.
Objective To investigate the degrees of acute impairment of the sertoli cell structure and function caused by different doses of 60Co-γ in mice. Methods Forty male KM mice were divided randomly into the control group (group A, n=10), and three bilateral testicular 60Co-γ radiation groups, such as the 2 Gy group (group B,n=10) , the 6 Gy group (group C, n=10), the 10 Gy group (group D, n=10). The lower abdomen and scrotal area of mice were irradiated with 60Co-γ ray for 517s in the three radiation groups. The levels of serum follicle-stimulating hormone (FSH), luteinizing hormone (LH), and inhibin β were determined by ELISA, the structural changes of sertoli cell were observed by light microscope, and the relative expressions of Inhibin β mRNA in the testicular tissues were detected by Real-time PCR 48 h after the radiation. Results Compared with that of group A,the serum FSH levels in groups B, C and D were significantly inctreased(P<0.05), and the serum Inhibin β levels were significantly decreased (P<0.05). The injured sertoli cells and spermatogenic cells were observed in all three 60Co-γ radiation groups, especially the most severe in the group D. The mRNA expression of Inhibin β in groups B, C and D were significantly lower than that in group A (P<0.05). Conclusion 60Co-γ irradiation might injury sertoli cell structure and function in mice, andthe irradiation dose was positively correlated with injury degree .
