CAJ | 학술논문

目的探讨5α还原酶抑制剂对人前列腺增生组织中 IGF-1、PCNA、Caspase3的影响及相关性.方法采用免疫组化法(PV9000法)检测良性前列腺增生患者服5α还原酶抑制剂组(简称服药组)与未服药组 IGF-1及 PCNA、Caspase3的表达,并分析三者间的相关性.结果(1)前列腺增生患者服药组 IGF-1阳性表达率及 PCNA 表达较未服药组表达明显减弱(P <0.05),Caspase3阳性率表达在服药组明显增加(P <0.05).服药组尿道周 IGF-1和 PCNA 的表达强于包膜下部位的趋势,而 Caspase3弱于包膜下,但差异均无统计学意义(P >0.05).(2)5α还原酶抑制剂作用下 I GF -1,P C N A 和 Caspase3的相关性表达研究显示,IGF-1与 PCNA 呈正相关(P <0.01),IGF-1与 Caspase3的表达呈负相关(P <0.05).结论(1)5α还原酶抑制剂与 IGF-1密切相关,可能通过抑制 IGF-1途径抑制细胞增殖和促进凋亡.(2)5α还原酶抑制剂对前列腺增生包膜下及尿道周抑制作用存在差异,可能是不能完全抑制前列腺细胞增生和促进凋亡的原因.
목적탐토5α환원매억제제대인전렬선증생조직중 IGF-1、PCNA、Caspase3적영향급상관성.방법채용면역조화법(PV9000법)검측량성전렬선증생환자복5α환원매억제제조(간칭복약조)여미복약조 IGF-1급 PCNA、Caspase3적표체,병분석삼자간적상관성.결과(1)전렬선증생환자복약조 IGF-1양성표체솔급 PCNA 표체교미복약조표체명현감약(P <0.05),Caspase3양성솔표체재복약조명현증가(P <0.05).복약조뇨도주 IGF-1화 PCNA 적표체강우포막하부위적추세,이 Caspase3약우포막하,단차이균무통계학의의(P >0.05).(2)5α환원매억제제작용하 I GF -1,P C N A 화 Caspase3적상관성표체연구현시,IGF-1여 PCNA 정정상관(P <0.01),IGF-1여 Caspase3적표체정부상관(P <0.05).결론(1)5α환원매억제제여 IGF-1밀절상관,가능통과억제 IGF-1도경억제세포증식화촉진조망.(2)5α환원매억제제대전렬선증생포막하급뇨도주억제작용존재차이,가능시불능완전억제전렬선세포증생화촉진조망적원인.
Abscract Objective To investigate the effects of 5α-reductase inhibitor on the expressions of IGF-1(insulin-like factor-1), PCNA(proliferation cell nuclear antigen) and Caspase3 in human prostatic hyperplastic tissues and its relationship. Methods The expressions of PCNA, Caspase3, IGF-1 were detected by immunohistochemical analysis in prostate tissues of BPH patients treated with 5α-reductase inhibitor.and the relationships among PCNA, Caspase3, IGF-1 were analyzied. Results (1) The positive rate of IGF-1 and PCNA in the treated group with 5 α -reductase inhibitor were markedly lower than those of the untreated group ( P<0.05). There was significant difference in the positive rate of Caspase3 in two groups.The tendency of IGF-1and PCNA in periurethal tissues higher than those of under amicula in treated BPH group was discovered,Caspase3 lower than those of under amicula, but there was no statistically significant. (2) There was a positive correlation between IGF-1 and PCNA(P<0.01).On the contrary, the expression of IGF-1 was negatively correlated with the expression of Caspase3 in the treated group( P<0.05). Conclusion (1)5α-reductase inhibitor might reduce cell proliferation and enhance cell apoptosis by inhibiting the expression of IGF-1 ; (2)A difference was found in inhibition effect on different BPH sites by 5 α -reductase inhibitor,which was probably associated with incompletely inhibition of prostatic cell proliferation and cell apoptosis increase.