CAJ | 학술논문

目的探讨前列舒通胶囊对前列腺增生抑制作用的机制.方法采用去势和注射丙酸睾酮的方法复制前列腺增生大鼠模型,将动物分为6组:正常对照组、模型对照组、前列舒通胶囊大(0.6 g / kg )、中(0.3g/kg )、小剂量(0.15g/kg )组和保列治组(0.8mg/kg ),连续给药4周,观察大鼠的前列腺湿质量、前列腺指数和病理组织形态、微血管密度,以及血清与前列腺组织 DHT、T 及 E2的含量,VEGF 的变化.采用 ELISA 法检测 DHT 的含量,体外观察前列舒通胶囊对前列腺5-α还原酶活性的作用.结果前列舒通胶囊能明显降低前列腺湿质量与前列腺指数,改善前列腺组织增生病理;明显降低血清与前列腺组织 DHT 与 T 的含量,升高血清与前列腺组织 E2含量;明显降低前列腺组织微血管密度,降低 VEGF 的表达.前列舒通胶囊体外能浓度依赖性的抑制 DHT 的生成,其最大抑制率为54.70%.结论前列舒通胶囊具有明显的抑制前列腺增生的作用,其作用机制与其抑制雄激素代谢和血管新生有关.
목적탐토전렬서통효낭대전렬선증생억제작용적궤제.방법채용거세화주사병산고동적방법복제전렬선증생대서모형,장동물분위6조:정상대조조、모형대조조、전렬서통효낭대(0.6 g / kg )、중(0.3g/kg )、소제량(0.15g/kg )조화보렬치조(0.8mg/kg ),련속급약4주,관찰대서적전렬선습질량、전렬선지수화병리조직형태、미혈관밀도,이급혈청여전렬선조직 DHT、T 급 E2적함량,VEGF 적변화.채용 ELISA 법검측 DHT 적함량,체외관찰전렬서통효낭대전렬선5-α환원매활성적작용.결과전렬서통효낭능명현강저전렬선습질량여전렬선지수,개선전렬선조직증생병리;명현강저혈청여전렬선조직 DHT 여 T 적함량,승고혈청여전렬선조직 E2함량;명현강저전렬선조직미혈관밀도,강저 VEGF 적표체.전렬서통효낭체외능농도의뢰성적억제 DHT 적생성,기최대억제솔위54.70%.결론전렬서통효낭구유명현적억제전렬선증생적작용,기작용궤제여기억제웅격소대사화혈관신생유관.
Objective To investigate the mechanism of suppressing prostate hyperplasia in rats with Qianlieshutong(QLST) Capsule. Methods SD rats with prostate hyperplasia were first established by castration and testosterone injection, and then they were divided into six groups. QLST Capsule (0.15, 0.3 and 0.6 g/kg, p.o.) and finasteride (0.8mg/kg, p.o.) were administered for 4 weeks respectively. The wet weight of prostate ,the prostate index (PI) and histopathological morpology, the microvessels density(MVD) and level of E 2, DHT, T and VEGF were detected respectively. 5-αreducase activity were measured by ELASA. Results For rats in the treatment group with QLST Capsule, the levels of prostate wet weight, PI, MVD, VEGF expression in prostate, DHT and T in serum and prostate tissue, were all downregulated, whereas the level of E 2 in serum and prostate tissue was upregulated, and the histopathological changes in prostate were ameliorated, 5- α reducase activity was inhibited. Conclusion QLST Capsule could inhibit prostate hyperplasia, which might be related to restrain androgen and angiogenesis.