CAJ | 학술논문

目的分析Lyzl6基因在小鼠中的表达特点.方法本研究通过RT-PCR实验明确Lyzl6基因在9、15、18、21、28、35d和6月龄小鼠睾丸中的表达变化及Lyzl6基因在成年小鼠各组织表达特点.采用多种生物信息学方法对该基因进行生物信息学分析.结果 RT-PCR结果显示,Lyzl6基因在小鼠21d龄及之前的睾丸中没有表达,28d睾丸中开始持续高表达.Lyzl6基因在14种小鼠组织中呈现睾丸显著高表达.经生物信息学分析,Lyzl6蛋白属于典型的C型溶菌酶,是一种分泌蛋白,其蛋白的信号肽剪切位点在第19~20个氨基酸之间.小鼠和人Lyzl6蛋白氨基酸序列有高度相似性和同源性,显示该基因在哺乳动物中高度保守.结论Lyzl6基因为小鼠睾丸年龄依赖性表达基因,在小鼠睾丸中高表达,显示其可能在生精过程发挥重要作用.
목적분석Lyzl6기인재소서중적표체특점.방법본연구통과RT-PCR실험명학Lyzl6기인재9、15、18、21、28、35d화6월령소서고환중적표체변화급Lyzl6기인재성년소서각조직표체특점.채용다충생물신식학방법대해기인진행생물신식학분석.결과 RT-PCR결과현시,Lyzl6기인재소서21d령급지전적고환중몰유표체,28d고환중개시지속고표체.Lyzl6기인재14충소서조직중정현고환현저고표체.경생물신식학분석,Lyzl6단백속우전형적C형용균매,시일충분비단백,기단백적신호태전절위점재제19~20개안기산지간.소서화인Lyzl6단백안기산서렬유고도상사성화동원성,현시해기인재포유동물중고도보수.결론Lyzl6기인위소서고환년령의뢰성표체기인,재소서고환중고표체,현시기가능재생정과정발휘중요작용.
Objective To investigate the expression characteristics of Lyzl6 in mouse with different postnatal days. Methods Testes tissue samples and other tissue samples were collected respectively from C57BL/6J mice with different postnatal days. Expressions of Lyzl6 gene in testes tissue and other tissue were identified by RT-PCR. The expression characteristic of Lyzl6 gene and the related bioinformatics analysis were done. Results RT-PCR assay showed that Lyzl6 gene high expressed in mouse testis, and Lyzl6 gene was persistently high expression in testis of the mouse from 28 days to 6 months after birth. Bioinformatics analysis showed that L yzl6 was a member of c-type lysozymes family, and it was a secretory protein, its’ signal peptide cleavage site was between position 19 and 20 amino acids. Lyzl6 protein amino acid sequence of human and mouse had a high degree of similarity and homology, indicated that this gene was highly conserved in mammals. Conclusion Lyzl6 gene was a age-dependent expression gene. and it’shigh expression in mouse testis suggested that it might play an important role in spermatogenesis.