CAJ | 학술논문

目的探讨多次低剂量X线照射对小鼠睾丸组织形态及生精细胞P53和bcl-2蛋白表达的影响.方法采用0、0.025、0.075及0.1Gy X线多次照射小鼠,以HE染色观察睾丸生精小管形态结构,免疫组织化学法测定生精细胞中P53及bcl-2蛋白的表达水平.结果随着单次低剂量X线照射剂量增加,HE染色结果显示照射组小鼠睾丸出现生精小管各级生精细胞数目逐渐减少,结构排列紊乱等不同程度的组织病理改变;免疫组织化学可见生精细胞中P53蛋白表达水平逐渐增加,而bcl-2蛋白表达水平则逐渐下降.结论低剂量X线多次全身照射可引起小鼠睾丸组织病理改变,并上调生精细胞P53蛋白、下调bcl-2蛋白表达水平,从而诱导凋亡.
목적탐토다차저제량X선조사대소서고환조직형태급생정세포P53화bcl-2단백표체적영향.방법채용0、0.025、0.075급0.1Gy X선다차조사소서,이HE염색관찰고환생정소관형태결구,면역조직화학법측정생정세포중P53급bcl-2단백적표체수평.결과수착단차저제량X선조사제량증가,HE염색결과현시조사조소서고환출현생정소관각급생정세포수목축점감소,결구배렬문란등불동정도적조직병리개변;면역조직화학가견생정세포중P53단백표체수평축점증가,이bcl-2단백표체수평칙축점하강.결론저제량X선다차전신조사가인기소서고환조직병리개변,병상조생정세포P53단백、하조bcl-2단백표체수평,종이유도조망.
Objective To investigate the effects of low-dose X-ray radiation on the testicular histomorphology and the expressions of P53 and bcl-2 protein in mouse spermatogenic cells. Methods Mice were exposed to repeated irradiation by 0, 0.025, 0.075 and 0.1Gy X-ray respectively. The morphology of testicular seminiferous tubules was observed using Hematoxylin-Eosin(HE) staining and the expressions of P53 and bcl-2 protein in germ cells were detected by immunohistochemistry. Results HE staining showed that the number of spermatogenic cells was gradually decreased in irradiated seminiferous tubules of mice testis and structural arrangement was destoryed in varying degrees in parallel with increase of single low-dose X-ray radiation dose .Immunohistochemical staining showed that the expression of P53 gradually was increased whereas the bcl-2 protein level was decreased in spermatogenic cells as increase of the single low-dose X-ray radiation dose. Conclusion Repeated low-dose X-ray irradiation might injury the histomorphology of testis, upregulate the expression of P53 and downregulate expression of Bcl2 in the rat testes, which may further induce germ cell apoptosis.