CAJ | 학술논문

转基因抗草甘膦作物是商业化最广泛的转基因作物之一,但是棉花的雄蕊对草甘膦敏感,在四叶期后喷施草甘膦会造成抗草甘膦棉花雄性败育,不能正常授粉,最终导致产量降低.为降低草甘膦对棉花育性的不良影响,有针对性的构建了棉花生殖器官优势表达的arf1启动子、棉花草甘膦高效诱导表达的ag2启动子和组成型启动子CaMV35S驱动苘麻epsps优化基因的串联三表达盒植物表达载体,采用农杆菌喷花法将其转入棉花,对T 0代喷施草甘膦筛选获得8株对草甘膦有较强抗性并且生殖发育正常的植株;PCR和RT-PCR分析表明外源基因已整合至棉花基因组并正确表达.为探索用不同类型启动子驱动相同基因以扩大外源基因在植物中的表达范围和培育具有自主知识产权的抗草甘膦转基因棉花打下基础.
전기인항초감련작물시상업화최엄범적전기인작물지일,단시면화적웅예대초감련민감,재사협기후분시초감련회조성항초감련면화웅성패육,불능정상수분,최종도치산량강저.위강저초감련대면화육성적불량영향,유침대성적구건료면화생식기관우세표체적arf1계동자、면화초감련고효유도표체적ag2계동자화조성형계동자CaMV35S구동경마epsps우화기인적천련삼표체합식물표체재체,채용농간균분화법장기전입면화,대T 0대분시초감련사선획득8주대초감련유교강항성병차생식발육정상적식주;PCR화RT-PCR분석표명외원기인이정합지면화기인조병정학표체.위탐색용불동류형계동자구동상동기인이확대외원기인재식물중적표체범위화배육구유자주지식산권적항초감련전기인면화타하기출.
@@@@Genetically modified glyphosate-resistant (GR)crops are widely commercialized GM crops in the world. However,cotton stamens are sensitive to glyphosate treatment,resulting in poor pollination and boll abortion and less cotton yield. To further improve the glyphosate-resistance trait of cotton and reproductive abnormalities in GR cotton, we employed a strategy in which the optimized epsps gene from Abutilon theophrasti Medic were driven by 3 promoters of tandem coexpression,including constitutive promoter CaMV35S,cotton reproductive organs referential expression promoter arf1 and glyphosate-induced promoter ag2. The construct was introduced into cotton via Agrobacterium based flower dipping approach. Eight T0 generation glyphosate-resistant plants were obtained after glyphosate screening,and their reproductive development were normal. PCR and RT-PCR results suggested that the epsps gene had successfully integrated into the cotton genome and had expressed correctly. These results indicated the possibilities of broadening the expression scope about a gene promoted by different types of promoters,so as to obtaining our own genetically modified GR cotton.