中国农业科技导报
中國農業科技導報
중국농업과기도보
REVIEW OF CHINA AGRICULTURAL SCIENCE AND TECHNOLOGY
2013年
1期
76-83
,共8页
王靓%李树%毛忠贵*%赵福林
王靚%李樹%毛忠貴*%趙福林
왕정%리수%모충귀*%조복림
ε-聚赖氨酸(ε-PL)%培养基%响应面分析%优化
ε-聚賴氨痠(ε-PL)%培養基%響應麵分析%優化
ε-취뢰안산(ε-PL)%배양기%향응면분석%우화
ε-polylysine(ε-PL)%culture medium%response surface methodology%optimization
对Streptomyces sp. FEEL-1产ε-聚赖氨酸的发酵培养基(M3G)中的6种主要成分进行了PB-CCD的响应面优化.PB实验表明,酵母粉、KH 2 PO4和MgSO 4对ε-PL产量影响显著,结合最陡爬坡试验和中心组合实验构建出各因素的最佳浓度:葡萄糖50 g/ L,酵母粉7.54 g/ L,(NH 4)2 SO45 g/ L,KH 2 PO41 g/ L,K 2 HPO42.48 g/ L,MgSO 42 g/ L,ZnSO 40.03 g/ L,FeSO 40.03 g/ L,该条件下ε-PL摇瓶产量能达1.62 g/ L.在此基础上对微量元素ZnSO 4,FeSO 4进行了单因素实验的优化,发现ZnSO 4对ε-PL 产量几乎没有影响,而0.06 g/ L的FeSO4则可以进一步使ε-PL产量提高至1.73 g/ L,较M3G培养基提高了57.2%.在5 L发酵罐中进行控制pH的分批发酵,ε-PL产量达到8.76 g/ L,较其在M3G培养基提高了41.1%.
對Streptomyces sp. FEEL-1產ε-聚賴氨痠的髮酵培養基(M3G)中的6種主要成分進行瞭PB-CCD的響應麵優化.PB實驗錶明,酵母粉、KH 2 PO4和MgSO 4對ε-PL產量影響顯著,結閤最陡爬坡試驗和中心組閤實驗構建齣各因素的最佳濃度:葡萄糖50 g/ L,酵母粉7.54 g/ L,(NH 4)2 SO45 g/ L,KH 2 PO41 g/ L,K 2 HPO42.48 g/ L,MgSO 42 g/ L,ZnSO 40.03 g/ L,FeSO 40.03 g/ L,該條件下ε-PL搖瓶產量能達1.62 g/ L.在此基礎上對微量元素ZnSO 4,FeSO 4進行瞭單因素實驗的優化,髮現ZnSO 4對ε-PL 產量幾乎沒有影響,而0.06 g/ L的FeSO4則可以進一步使ε-PL產量提高至1.73 g/ L,較M3G培養基提高瞭57.2%.在5 L髮酵罐中進行控製pH的分批髮酵,ε-PL產量達到8.76 g/ L,較其在M3G培養基提高瞭41.1%.
대Streptomyces sp. FEEL-1산ε-취뢰안산적발효배양기(M3G)중적6충주요성분진행료PB-CCD적향응면우화.PB실험표명,효모분、KH 2 PO4화MgSO 4대ε-PL산량영향현저,결합최두파파시험화중심조합실험구건출각인소적최가농도:포도당50 g/ L,효모분7.54 g/ L,(NH 4)2 SO45 g/ L,KH 2 PO41 g/ L,K 2 HPO42.48 g/ L,MgSO 42 g/ L,ZnSO 40.03 g/ L,FeSO 40.03 g/ L,해조건하ε-PL요병산량능체1.62 g/ L.재차기출상대미량원소ZnSO 4,FeSO 4진행료단인소실험적우화,발현ZnSO 4대ε-PL 산량궤호몰유영향,이0.06 g/ L적FeSO4칙가이진일보사ε-PL산량제고지1.73 g/ L,교M3G배양기제고료57.2%.재5 L발효관중진행공제pH적분비발효,ε-PL산량체도8.76 g/ L,교기재M3G배양기제고료41.1%.
@@@@PB-CCD response surface methodology was applied to optimize the 6 major compounds of theε-polylysine-producing fermentation medium (M3G)for Streptomyces sp. FEEL-1. PB experiment showed yeast extract,K 2 HPO4 and MgSO4 played important roles for ε-PL production. Combining with the results of the steep ascent test and the central composite design,the optimum concentrations of factors were determined as follows:glucose 50 g/ L,yeast extract 7. 54 g/ L,(NH 4 ) 2 SO4 5 g/ L,KH 2 PO4 1 g/ L,K 2 HPO4 2. 48 g/ L,MgSO 4 2 g/ L, ZnSO4 0. 03 g/ L,FeSO 4 0. 03 g/ L. Under such condition,the concentration of ε-PL in shake flask increased to 1. 62 g/ L. On the basis of above result,single-factor experiment was employed to optimize the trace elements ZnSO 4 and FeSO4 . ZnSO4 showed no influence on the production of ε-PL while 0. 06 g/ L of FeSO4 could increase the production of ε-PL to 1. 73 g/ L,which was 57. 2% higher than that in M3G medium. The ε-PL yield reached to 8. 76 g/ L in 5L fermenter by controlling pH,which was increased by 41. 1 % compared to that in M3G medium.