CAJ | 학술논문

为了研究C1qC基因在草鱼(Ctenopharyngodon idella)免疫过程中所起的作用,利用RT-PCR和RACE方法克隆获得了 C1qC 基因 cDNA 全长序列,经序列分析表明,所克隆的 C1qC cDNA 全长为916 bp,包括开放阅读框(open reading frame, ORF)735 bp,5′端非编码区(untranslated region, UTR)89 bp和3′端非编码区(UTR)92 bp.735 bp的ORF共编码244个氨基酸,相对分子量为26162.5 U.同源性分析表明,草鱼与斑马鱼(Danio rerio)的相似度最高,达到71%.经草鱼呼肠孤病毒(grass carp reovirus, GCRV)诱导后,草鱼C1qC基因在鳃、皮肤、肌肉、肝、中肾、心脏、头肾等组织中的mRNA表达水平均显著上调.在草鱼胚胎发育的各个阶段都能检测到C1qC mRNA的表达,说明该基因可能在草鱼胚胎和鱼苗的免疫反应和早期发育中起重要作用.本研究将为今后在草鱼免疫功能方面深入研究C1qC基因提供基础资料.
위료연구C1qC기인재초어(Ctenopharyngodon idella)면역과정중소기적작용,이용RT-PCR화RACE방법극륭획득료 C1qC 기인 cDNA 전장서렬,경서렬분석표명,소극륭적 C1qC cDNA 전장위916 bp,포괄개방열독광(open reading frame, ORF)735 bp,5′단비편마구(untranslated region, UTR)89 bp화3′단비편마구(UTR)92 bp.735 bp적ORF공편마244개안기산,상대분자량위26162.5 U.동원성분석표명,초어여반마어(Danio rerio)적상사도최고,체도71%.경초어호장고병독(grass carp reovirus, GCRV)유도후,초어C1qC기인재새、피부、기육、간、중신、심장、두신등조직중적mRNA표체수평균현저상조.재초어배태발육적각개계단도능검측도C1qC mRNA적표체,설명해기인가능재초어배태화어묘적면역반응화조기발육중기중요작용.본연구장위금후재초어면역공능방면심입연구C1qC기인제공기출자료.
We evaluated the immune function of C1qC in grass carp (Ctenopharyngodon idella). The full-length C1qC cDNA was successfully cloned by RT-PCR and rapid amplification of cDNA ends (RACE). The full-length C1qC cDNA was 916 bp, consisting of a 735 bp open reading frame (ORF) encoding 244 amino acids, a 89 bp 5′untranslated region (5′UTR), and a 92 bp 3′untranslated region (3′UTR). The molecular weight of this mature peptide was estimated to be 26 162.5U. Multiple alignment analysis revealed that grass carp C1qC shares the highest identity with zebrafish C1qC (71%). We constructed a phylogenetic tree using the neighbor-Joining (NJ) method. Grass carp were grouped most closely with zebrafish (Danio rerio). The expression of grass carp C1qC was significantly up-regulated in most tissues following challenge with grass carp reovirus (GCRV). The expres-sion of C1qC expression differed during the various stages of embryonic development. Our results suggest that C1qC plays an important role in early embryonic development and in the response to GCRV-related diseases in grass carp. Our result provide a basis for further evaluation of the role of grass carp C1qC in immune function.